Partial Sequence Analysis of Commercial Peste des Petits Ruminants Vaccines Produced in Africa

Peste des petits ruminants virus (PPRV), which is the only member of the species and belongs to the genus within the family, causes the highly contagious viral sickness "Peste des petits ruminants (PPR)." PPR is of serious economic significance for small ruminant production, particularly i...

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Published in:Veterinary sciences 2024-10, Vol.11 (10), p.500
Main Authors: Barry, Boubacar, Tessema, Yebechaye, Gelaw, Hassen, Boukary, Cisse Rahamatou Moustapha, Dieu, Baziki Jean de, Gelagay, Melesse Ayelet, Chitsungo, Ethel, Sanga, Richard Rayson, Oladosu, Gbolahanmi Akinola, Nwankpa, Nick, Bodjo, S Charles
Format: Article
Language:English
Subjects:
Biological products industry
C-Terminus
Eradication
Genes
Genetic analysis
genetic stability
Genomes
Homology
Immunization
Information management
master seed
molecular evolution
Morbillivirus
Mutation
Nucleotide sequence
Peste des petits ruminants
Proteins
Quality control
Sequence analysis
Sheep
Vaccination
vaccine
Vaccines
Viral diseases
Viruses
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Summary:Peste des petits ruminants virus (PPRV), which is the only member of the species and belongs to the genus within the family, causes the highly contagious viral sickness "Peste des petits ruminants (PPR)." PPR is of serious economic significance for small ruminant production, particularly in Africa. Control of this critical disease depends highly on successful vaccination against the PPRV. An in-depth understanding of the genetic evolution of the live-attenuated PPR vaccine Nigeria 75/1 strain used in Africa is essential for the successful eradication of this disease by 2030. Therefore, this study investigated the possible genetic evolution of the PPR vaccine produced by various African laboratories compared with the master seed available at AU-PANVAC. RT-PCR was performed to amplify a segment of the hypervariable C-terminal part of the nucleoprotein (N) from commercial batches of PPR vaccine Nigeria 75/1 strain. The sequences were analyzed, and 100% nucleotide sequence identity was observed between the master seed and vaccines produced. The results of this study indicate the genetic stability of the PPR vaccine from the Nigeria 75/1 strain over decades and that the vaccine production process used by different manufacturers did not contribute to the emergence of mutations in the vaccine strain.
ISSN:2306-7381
2306-7381
DOI:10.3390/vetsci11100500