Ciprofloxacin loaded PEG coated ZnO nanoparticles with enhanced antibacterial and wound healing effects

• Published in: Scientific reports 2024-02, Vol.14 (1), p.4689-18, Article 4689
• Main Authors: Ibne Shoukani, Hussan, Nisa, Sobia, Bibi, Yamin, Zia, Muhammad, Sajjad, Anila, Ishfaq, Afsheen, Ali, Hussain
• Format: Article
• Language: English
• Subjects: 631/1647; 631/326; 631/61; 639/301; Anti-Bacterial Agents - chemistry; Anti-Bacterial Agents - pharmacology; Antibacterial; Antibiotics; Antimicrobial resistance; Broad-spectrum; Cell lines; CIP-PEG-ZnO-NPs; Ciprofloxacin; Ciprofloxacin - pharmacology; Cytotoxic effects; Drug delivery; Drug delivery systems; Fourier transforms; Humanities and Social Sciences; Infrared spectroscopy; Metal Nanoparticles - chemistry; Microbial Sensitivity Tests; Microscopy, Electron, Scanning; multidisciplinary; Nano-drug delivery system; Nanocomposites; Nanoparticles; Nanoparticles - chemistry; Plant Extracts - chemistry; Plant Extracts - pharmacology; Polyethylene glycol; Scanning electron microscopy; Science; Science (multidisciplinary); Spectroscopy, Fourier Transform Infrared; Wound healing; Zinc oxide; Zinc Oxide - chemistry; Zinc oxides
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-55306-z

Antimicrobial resistance is a worldwide health problem that demands alternative antibacterial strategies. Modified nano-composites can be an effective strategy as compared to traditional medicine. The current study was designed to develop a biocompatible nano-drug delivery system with increased efficacy of current therapeutics for biomedical applications. Zinc oxide nanoparticles (ZnO-NPs) were synthesized by chemical and green methods by mediating with Moringa olifera root extract. The ZnO–NPs were further modified by drug conjugation and coating with PEG (CIP-PEG-ZnO-NPs) to enhance their therapeutic potential. PEGylated ZnO-ciprofloxacin nano-conjugates were characterized by Fourier Transform Infrared spectroscopy, X-ray diffractometry, and Scanning Electron Microscopy. During antibacterial screenings chemically and green synthesized CIP-PEG-ZnO-NPs revealed significant activity against clinically isolated Gram-positive and Gram-negative bacterial strains. The sustainable and prolonged release of antibiotics was noted from the CIP–PEG conjugated ZnO-NPs. The synthesized nanoparticles were found compatible with RBCs and Baby hamster kidney cell lines (BHK21) during hemolytic and MTT assays respectively. Based on initial findings a broad-spectrum nano-material was developed and tested for biomedical applications that eradicated Staphylococcus aureus from the infectious site and showed wound-healing effects during in vivo applications. ZnO-based nano-drug carrier can offer targeted drug delivery, and improved drug stability and efficacy resulting in better drug penetration.