Characterization of phosphomevalonate kinase: chromosomal localization, regulation, and subcellular targeting

Phosphomevalonate kinase catalyzes the conversion of mevalonate-5-phosphate to mevalonate-5-diphosphate and was originally believed to be a cytosolic enzyme. In this study we have localized the phosphomevalonate kinase gene to chromosome 1p13–1q22–23 and present a genomic map indicating that the gen...

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Bibliographic Details
Published in:Journal of lipid research 1999-04, Vol.40 (4), p.672-679
Main Authors: Olivier, Lisa M., Chambliss, Ken L., Michael Gibson, K., Krisans, Skaidrite K.
Format: Article
Language:English
Subjects:
3-hydroxy-3-methylglutaryl CoA
Amino Acid Sequence
Animals
Chromosome Mapping
Chromosomes, Human, Pair 1
dietary sterols
Gene Expression Regulation, Enzymologic - drug effects
Gene Targeting
Humans
Liver - enzymology
Liver - ultrastructure
Male
Microbodies - enzymology
Molecular Sequence Data
peroxisomes
Phosphotransferases (Phosphate Group Acceptor) - chemistry
Phosphotransferases (Phosphate Group Acceptor) - genetics
Phosphotransferases (Phosphate Group Acceptor) - metabolism
Protein Sorting Signals - chemistry
Rats
Rats, Sprague-Dawley
RNA, Messenger - analysis
Sequence Alignment
Sterols - administration & dosage
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Summary:Phosphomevalonate kinase catalyzes the conversion of mevalonate-5-phosphate to mevalonate-5-diphosphate and was originally believed to be a cytosolic enzyme. In this study we have localized the phosphomevalonate kinase gene to chromosome 1p13–1q22–23 and present a genomic map indicating that the gene spans more than 8.4 kb in the human genome. Furthermore, we show that message levels and enzyme activity of rat liver phosphomevalonate kinase are regulated in response to dietary sterol levels and that this regulation is coordinate with 3-hydroxy-3-methylglutaryl coenzyme A reductase, the rate-limiting enzyme of cholesterol biosynthesis. In addition, we demonstrate that phosphomevalonate kinase is a peroxisomal protein which requires the C-terminal peroxisomal targeting signal, Ser-Arg-Leu, for localization to the organelle.—Olivier, L. M., K. L. Chambliss, K. M. Gibson, and S. K. Krisans. Characterization of phosphomevalonate kinase: chromosomal localization, regulation, and subcellular targeting. J. Lipid Res. 1999. 40: 672–679.
ISSN:0022-2275
1539-7262
DOI:10.1016/S0022-2275(20)32146-5