Transforming plant tissue culture with nanoparticles: A review of current applications

• Published in: Plant Nano Biology 2024-11, Vol.10, p.100102, Article 100102
• Main Authors: Gunasena, M.D.K.M., Alahakoon, A.M.P.D., Polwaththa, K.P.G.D.M., Galpaya, G.D.C.P., Priyanjani, H.A.S.A., Koswattage, K.R., Senarath, W.T.P.S.K.
• Format: Article
• Language: English
• Subjects: Callus induction; Cell culture; Nanoparticles; Plant tissue culture; Somatic embryogenesis; Surface sterilization; Toxicity
• ISSN: 2773-1111; 2773-1111
• DOI: 10.1016/j.plana.2024.100102

The integration of nanotechnology into plant tissue culture represents a significant advancement in agricultural biotechnology. This review explores the transformative potential of nanoparticles in enhancing various processes within plant tissue culture. This review discussed how nanoparticles improve micropropagation efficiency by reducing contaminations, improving callus induction and increasing yields of secondary metabolites through cell suspension cultures. Additionally, the positive effects of nanoparticles on organogenesis, somatic embryogenesis, protoplast cultures and somaclonal variations are reviewed. Various types of nanoparticles, including silver, gold, zinc, cobalt, silica, and carbon-based nanoparticles, are analyzed for their specific applications and mechanisms of action. However, the potential toxicity of nanoparticles and their impact on plant health and the environment are critical concerns that are also reviewed. This comprehensive review provides insights into current applications, advantages and challenges of nanoparticle use in plant tissue culture, emphasizing the need for further research to optimize these innovative approaches for sustainable agricultural practices. •Nanoparticles boost micropropagation by reducing contamination, promoting callus growth.•Applications of silver, gold, zinc, cobalt, silica, and carbon nanoparticles are revealed.•Nanoparticle toxicity in plant tissue culture demands more research to ensure safety.