Functional analysis of a Phytophthora host-translocated effector using the yeast model system

plant pathogens secrete effector proteins that are translocated into host plant cells during infection and collectively contribute to pathogenicity. A subset of these host-translocated effectors can be identified by the amino acid motif RXLR (arginine, any amino acid, leucine, arginine). Bioinformat...

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Bibliographic Details
Published in:PeerJ (San Francisco, CA) CA), 2021-12, Vol.9, p.e12576-e12576, Article e12576
Main Authors: Wilson, Avery C, Morgan, William R
Format: Article
Language:English
Subjects:
Amino acids
Analysis
Arginine
Bioinformatics
Effectors
Functional genomics
Gene expression
Genes
Genetic transcription
Genomes
Genomics
Health aspects
Homeostasis
Host plants
Infection
Infections
Molecular Biology
Mycology
Pathogenicity
Pathogens
Peptides
Phosphate homeostasis
Phosphates
Phytopathology
Phytophthora
Phytophthora sojae
Plant cells
Plant Science
Proteins
Saccharyomyces cerevisiae
Transcriptomes
Yeast
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Summary:plant pathogens secrete effector proteins that are translocated into host plant cells during infection and collectively contribute to pathogenicity. A subset of these host-translocated effectors can be identified by the amino acid motif RXLR (arginine, any amino acid, leucine, arginine). Bioinformatics analysis has identified hundreds of putative RXLR effector genes in genomes, but the specific molecular function of most remains unknown. Here we describe initial studies to investigate the use of as a eukaryotic model to explore the function of RXLR effector proteins. Expression of individual RXLR effectors in yeast inhibited growth, consistent with perturbation of a highly conserved cellular process. Transcriptome analysis of yeast cells expressing the poorly characterized RXLR effector Avh110 identified nearly a dozen yeast genes whose expression levels were altered greater than two-fold compared to control cells. All five of the most down-regulated yeast genes are normally induced under low phosphate conditions the PHO4 transcription factor, indicating that PsAvh110 perturbs the yeast regulatory network essential for phosphate homeostasis and suggesting likely PsAvh110 targets during infection of its soybean host.
ISSN:2167-8359
2167-8359
DOI:10.7717/peerj.12576