High-Throughput Sequencing Reveals Further Diversity of Little Cherry Virus 1 with Implications for Diagnostics

(LChV1, , ) is a widespread pathogen of sweet or sour cherry and other species, which exhibits high genetic diversity and lacks a putative efficient transmission vector. Thus far, four distinct phylogenetic clusters of LChV1 have been described, including isolates from different species. The recent...

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Bibliographic Details
Published in:Viruses 2018-07, Vol.10 (7), p.385
Main Authors: Katsiani, Asimina, Maliogka, Varvara I, Katis, Nikolaos, Svanella-Dumas, Laurence, Olmos, Antonio, Ruiz-García, Ana B, Marais, Armelle, Faure, Chantal, Theil, Sébastien, Lotos, Leonidas, Candresse, Thierry
Format: Article
Language:English
Subjects:
Closteroviridae
Closteroviridae - genetics
Closteroviridae - isolation & purification
diagnostics
Disease transmission
Fruit
Fruits
Genetic diversity
Genetic Variation
Genome, Viral
Genomes
High-Throughput Nucleotide Sequencing
high-throughput sequencing
intra-host variability
LChV1
Life Sciences
Microbiology and Parasitology
Next-generation sequencing
Pathogenicity
Pathogens
Phylogenetics
Phylogeny
Phytopathology and phytopharmacy
Plant Diseases - virology
Plant pathology
Proteins
Prunus
Prunus - virology
RNA polymerase
RNA, Viral - genetics
Sequence Analysis, DNA
Species
Taxonomy
Trees
Vegetal Biology
Virology
Virus Diseases - diagnosis
Viruses
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Summary:(LChV1, , ) is a widespread pathogen of sweet or sour cherry and other species, which exhibits high genetic diversity and lacks a putative efficient transmission vector. Thus far, four distinct phylogenetic clusters of LChV1 have been described, including isolates from different species. The recent application of high throughput sequencing (HTS) technologies in fruit tree virology has facilitated the acquisition of new viral genomes and the study of virus diversity. In the present work, several new LChV1 isolates from different countries were fully sequenced using different HTS approaches. Our results reveal the presence of further genetic diversity within the LChV1 species. Interestingly, mixed infections of the same sweet cherry tree with different LChV1 variants were identified for the first time. Taken together, the high intra-host and intra-species diversities of LChV1 might affect its pathogenicity and have clear implications for its accurate diagnostics.
ISSN:1999-4915
1999-4915
DOI:10.3390/v10070385