Chromosomal alterations detected by comparative genomic hybridization in subgroups of gene expression-defined Burkitt's lymphoma

• Published in: Haematologica (Roma) 2008-09, Vol.93 (9), p.1327-1334
• Main Authors: Salaverria, Itziar, Zettl, Andreas, Bea, Silvia, Hartmann, Elena M, Dave, Sandeep S, Wright, George W, Boerma, Evert-Jan, Kluin, Philip M, Ott, German, Chan, Wing C, Weisenburger, Dennis D, Lopez-Guillermo, Armando, Gascoyne, Randy D, Delabie, Jan, Rimsza, Lisa M, Braziel, Rita M, Jaffe, Elaine S, Staudt, Louis M, Muller-Hermelink, Hans Konrad, Campo, Elias, Rosenwald, Andreas, Leukemia and Lymphoma Molecular Profiling Project (LLMPP)
• Format: Article
• Language: English
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Biological and medical sciences; Burkitt Lymphoma - classification; Burkitt Lymphoma - genetics; Child; Child, Preschool; Chromosomes, Human - genetics; Comparative Genomic Hybridization; Female; Gene Expression Profiling; Gene Expression Regulation, Neoplastic - genetics; Hematologic and hematopoietic diseases; Humans; Infectious diseases; Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis; Male; Medical sciences; Middle Aged; Viral diseases
• ISSN: 0390-6078; 1592-8721
• DOI: 10.3324/haematol.13071

1 Depts. of Pathology, University of Barcelona, Barcelona, Spain 2 Institute of Pathology, University of Würzburg, Würzburg, Germany 3 Metabolism Branch 4 Biometric Research Branch, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA 5 Dept. of Pathology, University Medical Center Groningen, Groningen, The Netherlands 6 Dept. of Pathology and Microbiology, University of Nebraska Medical Center, Omaha, NE, USA 7 Hematology, Hospital Clínic, University of Barcelona, Barcelona, Spain 8 British Columbia Cancer Agency, Vancouver, B.C., Canada 9 Norwegian Radium Hospital, Norway Hospital Clinic, Oslo, Norway 10 Dept. of Pathology, University of Arizona, Tucson, AZ, USA 11 Dept. of Pathology, Oregon Health and Sciences University, Portland, OR, USA 12 Laboratory of Pathology, National Cancer Institute, National Institutes of Health, Bethesda, MD, USA 13 Institute of Clinical Pathology, Robert-Bosch-Krankenhaus, Stuttgart, Germany Correspondence: Andreas Rosenwald, Institute of Pathology, University of Würzburg, Josef-Schneider-Str. 2 97080 Würzburg, Germany Email: Rosenwald{at}mail.uni-wuerzburg.de Background: Burkitt’s lymphoma is an aggressive B-cell lymphoma characterized by typical morphological, immunophenotypic and molecular features. Gene expression profiling provided a molecular signature of Burkitt’s lymphoma, but also demonstrated that a subset of aggressive B-cell lymphomas not fulfilling the current World Health Organization criteria for the diagnosis of Burkitt’s lymphoma nonetheless show a molecular signature of Burkitt’s lymphoma (‘discrepant Burkitt’s lymphoma’). Given the different treatment of Burkitt’s lymphoma and diffuse large B-cell lymphomas we investigated molecular differences within gene expression-defined Burkitt’s lymphoma. Design and Methods: We studied tumors from 51 Burkitt’s lymphoma patients, comprising 26 with classic Burkitt’s lymphoma, 17 with atypical Burkitt’s lymphoma and 8 with ‘discrepant Burkitt’s lymphoma’, by comparative genomic hybridization and gene expression profiling. Results: Classic and atypical Burkitt’s lymphoma (excluding ‘discrepant Burkitt’s lymphoma’), in adult and pediatric cases do not differ in underlying genomic imbalances or gene expression suggesting that these subgroups are molecularly homogeneous. ‘Discrepant Burkitt’s lymphoma’, however, differ dramatically in the absolute number of alterations from classic/atypical Burkitt’s lymphoma and from diffuse large B-cell lymphoma.