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Microalgae to Bioenergy: Optimization of Aurantiochytrium sp. Saccharification
Microalgae are a promising feedstock for bioethanol production, essentially due to their high growth rates and absence of lignin. Hydrolysis-where the monosaccharides are released for further fermentation-is considered a critical step, and its optimization is advised for each raw material. The prese...
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Published in: | Biology (Basel, Switzerland) Switzerland), 2023-06, Vol.12 (7), p.935 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Microalgae are a promising feedstock for bioethanol production, essentially due to their high growth rates and absence of lignin. Hydrolysis-where the monosaccharides are released for further fermentation-is considered a critical step, and its optimization is advised for each raw material. The present study focuses on the thermal acid hydrolysis (with sulfuric acid) of
sp. through a response surface methodology (RSM), studying the effect of acid concentration, hydrolysis time and biomass/acid ratio on both sugar concentration of the hydrolysate and biomass conversion yield. Preliminary studies allowed to establish the range of the variables to be optimized. The obtained models predicted a maximum sugar concentration (18.05 g/L; R
= 0.990) after 90 min of hydrolysis, using 15% (
/
) biomass/acid ratio and sulfuric acid at 3.5% (
/
), whereas the maximum conversion yield (12.86 g/100 g; R
= 0.876) was obtained using 9.3% (
/
) biomass/acid ratio, maintaining the other parameters. Model outputs indicate that the biomass/acid ratio and time are the most influential parameters on the sugar concentration and yield models, respectively. The study allowed to obtain a predictive model that is very well adjusted to the experimental data to find the best saccharification conditions for the
sp. microalgae. |
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ISSN: | 2079-7737 2079-7737 |
DOI: | 10.3390/biology12070935 |