Determination of Zn2+ in Solid Pharmaceutical Dosage Forms by Means of Spectrophotometry in Micellar Media: Method Validation

In this study, a spectrophotometric method for the quantitative determination of zinc in a dietary supplement was validated. Zinc forms a ternary complex with Xylenol Orange and cetylpyridinium chloride in acetate buffer at pH = 5.5. The complex exhibits an absorption maximum at 560 nm. Linearity (r...

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Bibliographic Details
Published in:Applied sciences 2022-05, Vol.12 (9), p.4476
Main Authors: Rus, Luca Liviu, Juncan, Anca Maria, Crăciun, Veronica Isabela, Frum, Adina, Hegheș, Simona-Codruța, Butuca, Anca, Dobrea, Carmen Maximiliana, Chis, Adriana Aurelia, Muntean, Andrei Catalin, Vonica-Ţincu, Andreea Loredana, Morgovan, Claudiu
Format: Article
Language:English
Subjects:
AAS method
Absorption
Acetic acid
Acne
analytical method validation
Atomic absorption spectroscopy
Calibration
Cell division
Cetylpyridinium chloride
Chloride
Coefficient of variation
Confidence intervals
Correlation coefficient
Correlation coefficients
Dietary supplements
Dosage
Hydrochloric acid
Integrated software
Metabolism
Oxidative stress
pharmaceutical dosage forms
Pharmaceuticals
Potassium
Prostate
Quality control
Raw materials
Reagents
Scientific imaging
Spectral analysis
Spectrometry
Spectrophotometry
Zinc
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Summary:In this study, a spectrophotometric method for the quantitative determination of zinc in a dietary supplement was validated. Zinc forms a ternary complex with Xylenol Orange and cetylpyridinium chloride in acetate buffer at pH = 5.5. The complex exhibits an absorption maximum at 560 nm. Linearity (range was 0.5–1.5 µg/mL, and correlation coefficient of the calibration curve was 0.9998), precision (repeatability variation coefficient and reproducibility variation coefficient were 1.57% and 1.77%, respectively), and accuracy (confidence interval of the mean recovery was 98.29–100.55%) of this method fulfil current pharmacopeial requests. Results obtained by spectrophotometric method were statistically compared with ones obtained by atomic absorption spectrometry and good agreement was found. The method can be applied routinely for quality control of solid pharmaceutical dosage forms containing zinc.
ISSN:2076-3417
2076-3417
DOI:10.3390/app12094476