CXCL5 Modified Nanoparticle Surface Improves CXCR2 + Cell Selective Internalization

Driving nanomaterials to specific cell populations is still a major challenge for different biomedical applications. Several strategies to improve cell binding and uptake have been tried thus far by intrinsic material modifications or decoration with active molecules onto their surface. In the prese...

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Bibliographic Details
Published in:Cells (Basel, Switzerland) Switzerland), 2019-12, Vol.9 (1), p.56
Main Authors: Cagliani, Roberta, Gatto, Francesca, Cibecchini, Giulia, Marotta, Roberto, Catalano, Federico, Sanchez-Moreno, Paola, Pompa, Pier Paolo, Bardi, Giuseppe
Format: Article
Language:English
Subjects:
Amino acids
Chemokine receptors
Chemokines
Confocal microscopy
CXCR2 protein
Electron microscopy
Flow cytometry
immune cells
Internalization
Localization
Medical prognosis
Microscopy
Nanomaterials
Nanoparticles
Nanotechnology
Particle size
Penicillin
Peptides
Physiology
Proteins
Silicon dioxide
surface chemistry
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Summary:Driving nanomaterials to specific cell populations is still a major challenge for different biomedical applications. Several strategies to improve cell binding and uptake have been tried thus far by intrinsic material modifications or decoration with active molecules onto their surface. In the present work, we covalently bound the chemokine CXCL5 on fluorescently labeled amino-functionalized SiO nanoparticles to precisely targeting CXCR2 immune cells. We synthesized and precisely characterized the physicochemical features of the modified particles. The presence of CXCL5 on the surface was detected by z-potential variation and CXCL5-specific electron microscopy immunogold labeling. CXCL5-amino SiO nanoparticle cell binding and internalization performances were analyzed in CXCR2 THP-1 cells by flow cytometry and confocal microscopy. We showed improved internalization of the chemokine modified particles in the absence or the presence of serum. This internalization was reduced by cell pre-treatment with free CXCL5. Furthermore, we demonstrated CXCR2 cell preferential targeting by comparing particle uptake in THP-1 vs. low-CXCR2 expressing HeLa cells. Our results provide the proof of principle that chemokine decorated nanomaterials enhance uptake and allow precise cell subset localization. The possibility to aim at selective chemokine receptor-expressing cells can be beneficial for the diverse pathological conditions involving immune reactions.
ISSN:2073-4409
2073-4409
DOI:10.3390/cells9010056