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Single Cell and Single Nucleus RNA-Seq Reveal Cellular Heterogeneity and Homeostatic Regulatory Networks in Adult Mouse Stria Vascularis
The stria vascularis (SV) generates the endocochlear potential (EP) in the inner ear and is necessary for proper hair cell mechanotransduction and hearing. While channels belonging to SV cell types are known to play crucial roles in EP generation, relatively little is known about gene regulatory net...
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| Published in: | Frontiers in molecular neuroscience 2019-12, Vol.12, p.316-316 |
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| container_end_page | 316 |
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| container_title | Frontiers in molecular neuroscience |
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| creator | Korrapati, Soumya Taukulis, Ian Olszewski, Rafal Pyle, Madeline Gu, Shoujun Singh, Riya Griffiths, Carla Martin, Daniel Boger, Erich Morell, Robert J Hoa, Michael |
| description | The stria vascularis (SV) generates the endocochlear potential (EP) in the inner ear and is necessary for proper hair cell mechanotransduction and hearing. While channels belonging to SV cell types are known to play crucial roles in EP generation, relatively little is known about gene regulatory networks that underlie the ability of the SV to generate and maintain the EP. Using single cell and single nucleus RNA-sequencing, we identify and validate known and rare cell populations in the SV. Furthermore, we establish a basis for understanding molecular mechanisms underlying SV function by identifying potential gene regulatory networks as well as druggable gene targets. Finally, we associate known deafness genes with adult SV cell types. This work establishes a basis for dissecting the genetic mechanisms underlying the role of the SV in hearing and will serve as a basis for designing therapeutic approaches to hearing loss related to SV dysfunction. |
| doi_str_mv | 10.3389/fnmol.2019.00316 |
| format | article |
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While channels belonging to SV cell types are known to play crucial roles in EP generation, relatively little is known about gene regulatory networks that underlie the ability of the SV to generate and maintain the EP. Using single cell and single nucleus RNA-sequencing, we identify and validate known and rare cell populations in the SV. Furthermore, we establish a basis for understanding molecular mechanisms underlying SV function by identifying potential gene regulatory networks as well as druggable gene targets. Finally, we associate known deafness genes with adult SV cell types. This work establishes a basis for dissecting the genetic mechanisms underlying the role of the SV in hearing and will serve as a basis for designing therapeutic approaches to hearing loss related to SV dysfunction.</description><identifier>ISSN: 1662-5099</identifier><identifier>EISSN: 1662-5099</identifier><identifier>DOI: 10.3389/fnmol.2019.00316</identifier><identifier>PMID: 31920542</identifier><language>eng</language><publisher>Switzerland: Frontiers Research Foundation</publisher><subject>adult stria vascularis ; Deafness ; Ears & hearing ; Genomics ; hearing ; Hearing loss ; Inner ear ; Life sciences ; Mechanotransduction ; Molecular modelling ; Mutation ; Neuroscience ; Potassium ; regulons ; Ribonucleic acid ; RNA ; scRNA-Seq ; snRNA-Seq ; Stria vascularis</subject><ispartof>Frontiers in molecular neuroscience, 2019-12, Vol.12, p.316-316</ispartof><rights>Copyright © 2019 Korrapati, Taukulis, Olszewski, Pyle, Gu, Singh, Griffiths, Martin, Boger, Morell and Hoa.</rights><rights>2019. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Copyright © 2019 Korrapati, Taukulis, Olszewski, Pyle, Gu, Singh, Griffiths, Martin, Boger, Morell and Hoa. 2019 Korrapati, Taukulis, Olszewski, Pyle, Gu, Singh, Griffiths, Martin, Boger, Morell and Hoa</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c490t-fbc5341d909bb9f0a2abca5924c0d87add277af722a037472456698f10384b33</citedby><cites>FETCH-LOGICAL-c490t-fbc5341d909bb9f0a2abca5924c0d87add277af722a037472456698f10384b33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2328968442/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2328968442?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25731,27901,27902,36989,36990,44566,53766,53768,74869</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/31920542$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Korrapati, Soumya</creatorcontrib><creatorcontrib>Taukulis, Ian</creatorcontrib><creatorcontrib>Olszewski, Rafal</creatorcontrib><creatorcontrib>Pyle, Madeline</creatorcontrib><creatorcontrib>Gu, Shoujun</creatorcontrib><creatorcontrib>Singh, Riya</creatorcontrib><creatorcontrib>Griffiths, Carla</creatorcontrib><creatorcontrib>Martin, Daniel</creatorcontrib><creatorcontrib>Boger, Erich</creatorcontrib><creatorcontrib>Morell, Robert J</creatorcontrib><creatorcontrib>Hoa, Michael</creatorcontrib><title>Single Cell and Single Nucleus RNA-Seq Reveal Cellular Heterogeneity and Homeostatic Regulatory Networks in Adult Mouse Stria Vascularis</title><title>Frontiers in molecular neuroscience</title><addtitle>Front Mol Neurosci</addtitle><description>The stria vascularis (SV) generates the endocochlear potential (EP) in the inner ear and is necessary for proper hair cell mechanotransduction and hearing. 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This work establishes a basis for dissecting the genetic mechanisms underlying the role of the SV in hearing and will serve as a basis for designing therapeutic approaches to hearing loss related to SV dysfunction.</description><subject>adult stria vascularis</subject><subject>Deafness</subject><subject>Ears & hearing</subject><subject>Genomics</subject><subject>hearing</subject><subject>Hearing loss</subject><subject>Inner ear</subject><subject>Life sciences</subject><subject>Mechanotransduction</subject><subject>Molecular modelling</subject><subject>Mutation</subject><subject>Neuroscience</subject><subject>Potassium</subject><subject>regulons</subject><subject>Ribonucleic acid</subject><subject>RNA</subject><subject>scRNA-Seq</subject><subject>snRNA-Seq</subject><subject>Stria vascularis</subject><issn>1662-5099</issn><issn>1662-5099</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2019</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNpdkktvEzEUhUcIREthzwpZYsMmwY8Ze7xBiiIglUqQmoqtdcdzJ0xwxq3tKco_4GfjPKhaVn599_jeo1MUbxmdClHrj92w9W7KKdNTSgWTz4pzJiWfVFTr54_2Z8WrGDeUSi4r8bI4E0xzWpX8vPiz6oe1QzJH5wgMLTmdl6N1OEZyvZxNVnhHrvEewR2w0UEgC0wY_BoH7NPuULjwW_QxQeptpteZSj7syBLTbx9-RdIPZNaOLpFvfoxIVin0QH5AtHu9Pr4uXnTgIr45rRfFzZfPN_PF5Or718v57GpiS03TpGtsJUrWaqqbRncUODQWKs1LS9taQdtypaBTnAMVqlS8rKTUdceoqMtGiIvi8ijbetiY29BvIeyMh94cLnxYGwh5BIcGLHSirbhARUutVM3yzwIUbVnFZS2z1qej1u3YbLG1OKQA7ono05eh_2nW_t5ILQTlLAt8OAkEfzdiTGbbR5sthgGzSYYLIXlugKmMvv8P3fgxDNmpTPFay7oseabokbLBxxiwe2iGUbNPjDkkxuwTYw6JySXvHg_xUPAvIuIvZ0292A</recordid><startdate>20191220</startdate><enddate>20191220</enddate><creator>Korrapati, Soumya</creator><creator>Taukulis, Ian</creator><creator>Olszewski, Rafal</creator><creator>Pyle, Madeline</creator><creator>Gu, Shoujun</creator><creator>Singh, Riya</creator><creator>Griffiths, Carla</creator><creator>Martin, Daniel</creator><creator>Boger, Erich</creator><creator>Morell, Robert J</creator><creator>Hoa, Michael</creator><general>Frontiers Research Foundation</general><general>Frontiers Media S.A</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7TK</scope><scope>7XB</scope><scope>88I</scope><scope>8FE</scope><scope>8FH</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BHPHI</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>LK8</scope><scope>M2P</scope><scope>M7P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>Q9U</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20191220</creationdate><title>Single Cell and Single Nucleus RNA-Seq Reveal Cellular Heterogeneity and Homeostatic Regulatory Networks in Adult Mouse Stria Vascularis</title><author>Korrapati, Soumya ; Taukulis, Ian ; Olszewski, Rafal ; Pyle, Madeline ; Gu, Shoujun ; Singh, Riya ; Griffiths, Carla ; Martin, Daniel ; Boger, Erich ; Morell, Robert J ; Hoa, Michael</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c490t-fbc5341d909bb9f0a2abca5924c0d87add277af722a037472456698f10384b33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2019</creationdate><topic>adult stria vascularis</topic><topic>Deafness</topic><topic>Ears & hearing</topic><topic>Genomics</topic><topic>hearing</topic><topic>Hearing loss</topic><topic>Inner ear</topic><topic>Life sciences</topic><topic>Mechanotransduction</topic><topic>Molecular modelling</topic><topic>Mutation</topic><topic>Neuroscience</topic><topic>Potassium</topic><topic>regulons</topic><topic>Ribonucleic acid</topic><topic>RNA</topic><topic>scRNA-Seq</topic><topic>snRNA-Seq</topic><topic>Stria vascularis</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Korrapati, Soumya</creatorcontrib><creatorcontrib>Taukulis, Ian</creatorcontrib><creatorcontrib>Olszewski, Rafal</creatorcontrib><creatorcontrib>Pyle, Madeline</creatorcontrib><creatorcontrib>Gu, Shoujun</creatorcontrib><creatorcontrib>Singh, Riya</creatorcontrib><creatorcontrib>Griffiths, Carla</creatorcontrib><creatorcontrib>Martin, Daniel</creatorcontrib><creatorcontrib>Boger, Erich</creatorcontrib><creatorcontrib>Morell, Robert J</creatorcontrib><creatorcontrib>Hoa, Michael</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Neurosciences Abstracts</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Science Database (Alumni Edition)</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Natural Science Collection</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>ProQuest Central Student</collection><collection>SciTech Premium Collection</collection><collection>Biological Sciences</collection><collection>Science Database (ProQuest)</collection><collection>Biological Science Database</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>ProQuest Central Basic</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>Frontiers in molecular neuroscience</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Korrapati, Soumya</au><au>Taukulis, Ian</au><au>Olszewski, Rafal</au><au>Pyle, Madeline</au><au>Gu, Shoujun</au><au>Singh, Riya</au><au>Griffiths, Carla</au><au>Martin, Daniel</au><au>Boger, Erich</au><au>Morell, Robert J</au><au>Hoa, Michael</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Single Cell and Single Nucleus RNA-Seq Reveal Cellular Heterogeneity and Homeostatic Regulatory Networks in Adult Mouse Stria Vascularis</atitle><jtitle>Frontiers in molecular neuroscience</jtitle><addtitle>Front Mol Neurosci</addtitle><date>2019-12-20</date><risdate>2019</risdate><volume>12</volume><spage>316</spage><epage>316</epage><pages>316-316</pages><issn>1662-5099</issn><eissn>1662-5099</eissn><abstract>The stria vascularis (SV) generates the endocochlear potential (EP) in the inner ear and is necessary for proper hair cell mechanotransduction and hearing. 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| subjects | adult stria vascularis Deafness Ears & hearing Genomics hearing Hearing loss Inner ear Life sciences Mechanotransduction Molecular modelling Mutation Neuroscience Potassium regulons Ribonucleic acid RNA scRNA-Seq snRNA-Seq Stria vascularis |
| title | Single Cell and Single Nucleus RNA-Seq Reveal Cellular Heterogeneity and Homeostatic Regulatory Networks in Adult Mouse Stria Vascularis |
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