Fish Identification based on Partial Fragments of The Mitochondrial COI Subunit I Gene

The mtDNA sequences revealed that several of the fish studied were Hampala macrolepidota and Barbonymus gonionotus . The objective of this research was to learn the pattern of COI gene in mtDNA and establish a phylogenetic tree. Basic Local Alignment Search Tool-nucleotide (BLASTn) confirmed that Ba...

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Bibliographic Details
Published in:E3S Web of Conferences 2021, Vol.322, p.1038
Main Author: Wulandari, Tuah N. M.
Format: Article
Language:English
Subjects:
Barbonymus gonionotus
COI protein
Familiarity
Gene sequencing
Hampala macrolepidota
Matching
Mitochondria
Mitochondrial DNA
Nucleotides
Phylogeny
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Summary:The mtDNA sequences revealed that several of the fish studied were Hampala macrolepidota and Barbonymus gonionotus . The objective of this research was to learn the pattern of COI gene in mtDNA and establish a phylogenetic tree. Basic Local Alignment Search Tool-nucleotide (BLASTn) confirmed that Barbonymus gonionotus froma the Ranau Lake, South Sumatera has 100% matching ranges to the species from Memberamo River (Indonesia), India, Bangladesh, Thailand (Mae Khlong), Indo-Myanmar, and Malaysia_1. The lowest closeness (98.76%) is related to species from Thailand (Lower Ing). The Blast investigation appears us that the level of familiarity was very high, it is coming to 98-100% in Barbonymus gonionotus . Hampala macrolepidota had 100% matching ranges to the species from Indonesia (SouthaSumatera_1) and Vietnam. They had 99.05%-99.84% closeness from Malaysia_1,2&3, Indonesia (South Sumatera_2&3, Java and Bali_1,2&3).
ISSN:2267-1242
2555-0403
2267-1242
DOI:10.1051/e3sconf/202132201038