New methods for the quantification of mixed chimerism in transplantation

Quantification of chimerism showing the proportion of the donor in a recipient is essential for the follow-up of hematopoietic stem cell transplantation but can also be useful to document an immune tolerance situation after solid organ transplantation. Historically, chimerism has been quantified fro...

Full description

Saved in:
Bibliographic Details
Published in:Frontiers in immunology 2023-01, Vol.14, p.1023116-1023116
Main Authors: Picard, Christophe, Frassati, Coralie, Cherouat, Nicem, Maioli, Sandrine, Moskovtchenko, Philippe, Cherel, Mathilde, Chiaroni, Jacques, Pedini, Pascal
Format: Article
Language:English
Subjects:
Adaptive immunology
cfDNA (cell-free DNA)
Chimerism
dPCR (digital PCR)
Genetics
Hematopoietic Stem Cell Transplantation
Human genetics
Immunology
Innate immunity
Life Sciences
NGS (next generation sequencing)
Organ Transplantation
Polymerase Chain Reaction - methods
qPCR (quantitative PCR)
Transplantation Chimera - genetics
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Quantification of chimerism showing the proportion of the donor in a recipient is essential for the follow-up of hematopoietic stem cell transplantation but can also be useful to document an immune tolerance situation after solid organ transplantation. Historically, chimerism has been quantified from genomic DNA, but with technological advances, chimerism from donor-derived cell-free DNA seems particularly relevant in solid organ transplantation. The reference method was until recently the short tandem repeat technique, but new innovative techniques as digital PCR (dPCR) and NGS, have revolutionized the quantification of chimerism, such as the so-called microchimerism analysis. After a short review of chimerism methods, a comparison of chimerism quantification data for two new digital PCR systems (QIAcuity™ dPCR (Qiagen ) and QuantStudio Absolute Q (ThermoFisher ) and two NGS-based chimerism quantification methods (AlloSeq HCT™ (CareDx ) and NGStrack™ (GenDX )) was performed. These new methods were correlated and concordant to routinely methods (r²=0.9978 and r²=0.9974 for dPCR methods, r²=0.9978 and r²=0.9988 for NGS methods), and had similar high performance (sensitivity, reproductibility, linearity). Finally, the choice of the innovative method of chimerism within the laboratory does not depend on the analytical performances because they are similar but mainly on the amount of activity and the access to instruments and computer services.
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2023.1023116