In vitro activity of 1H-phenalen-1-one derivatives against Leishmania spp. and evidence of programmed cell death

• Published in: Parasites & vectors 2019-12, Vol.12 (1), p.601-601, Article 601
• Main Authors: López-Arencibia, Atteneri, Reyes-Batlle, María, Freijo, Mónica B, Sifaoui, Ines, Bethencourt-Estrella, Carlos J, Rizo-Liendo, Aitor, Chiboub, Olfa, McNaughton-Smith, Grant, Lorenzo-Morales, Jacob, Abad-Grillo, Teresa, Piñero, José E
• Format: Article
• Language: English
• Subjects: Antiprotozoal Agents - chemistry; Antiprotozoal Agents - pharmacology; Apoptosis; Apoptosis - drug effects; Apoptosis-like; Biochemistry; Cell death; Chemical compounds; Chemotherapy; Chromatin; Derivatives; Drugs; Humans; Leishmania; Leishmania - cytology; Leishmania - drug effects; Leishmania - growth & development; Leishmania amazonensis; Leishmania donovani; Leishmaniasis - parasitology; Life Cycle Stages - drug effects; Meglumine antimoniate; Membrane potential; Membranes; Mitochondria; Molecular Structure; Mortality; Novels; Oxidative stress; Parasites; Parasitic diseases; Pentamidine; Pharmacology; Phenalenes - chemistry; Phenalenes - pharmacology; Phenalenones; Phosphatidylserine; Phospholipids; Signal processing; Software; Tropical diseases; Variance analysis
• ISSN: 1756-3305; 1756-3305
• DOI: 10.1186/s13071-019-3854-4

The in vitro activity against Leishmania spp. of a novel group of compounds, phenalenone derivatives, is described in this study. Previous studies have shown that some phenalenones present leishmanicidal activity, and induce a decrease in the mitochondrial membrane potential in L. amazonensis parasites, so in order to elucidate the evidence of programmed cell death occurring inside the promastigote stage, different assays were performed in two different species of Leishmania. We focused on the determination of the programmed cell death evidence by detecting the characteristic features of the apoptosis-like process, such as phosphatidylserine exposure, mitochondrial membrane potential, and chromatin condensation among others. The results showed that four molecules activated the apoptosis-like process in the parasite. All the signals observed were indicative of the death process that the parasites were undergoing. The present results highlight the potential use of phenalenone derivatives against Leishmania species and further studies should be undertaken to establish them as novel leishmanicidal therapeutic agents.