Microbes translocation from oral cavity to nasopharyngeal carcinoma in patients

• Published in: Nature communications 2024-02, Vol.15 (1), p.1645-1645, Article 1645
• Main Authors: Liao, Ying, Wu, Yan-Xia, Tang, Minzhong, Chen, Yi-Wei, Xie, Jin-Ru, Du, Yan, Wang, Tong-Min, He, Yong-Qiao, Xue, Wen-Qiong, Zheng, Xiao-Hui, Liu, Qiao-Yun, Zheng, Mei-Qi, Jia, Yi-Jing, Tong, Xia-Ting, Zhou, Ting, Li, Xi-Zhao, Yang, Da-Wei, Diao, Hua, Jia, Wei-Hua
• Format: Article
• Language: English
• Subjects: 14/32; 45/77; 45/91; 49/22; 49/23; 631/326/2565/2134; 692/4028/67/1536; Biopsy; Cancer; Carcinogenesis; Carcinogens; Epstein-Barr virus; Humanities and Social Sciences; Microenvironments; Microorganisms; multidisciplinary; Nasopharyngeal carcinoma; Nasopharynx; Oral carcinoma; Oral cavity; Science; Science (multidisciplinary); Throat cancer; Transcriptomes; Translocation; Tumor microenvironment; Tumors
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-024-45518-2

The presence of oral microbes in extra-oral sites is linked to gastrointestinal cancers. However, their potential ectopically colonization in the nasopharynx and impact on local cancer development remains uncertain. Our study involving paired nasopharyngeal-oral microbial samples from nasopharyngeal carcinoma (NPC) patients and controls unveils an aberrant oral-to-nasopharyngeal microbial translocation associated with increased NPC risk (OR = 4.51, P  = 0.012). Thirteen species are classified as oral-translocated and enriched in NPC patients. Among these, Fusobacterium nucleatum and Prevotella intermedia are validated through culturomics and clonal strain identification. Nasopharyngeal biopsy meta-transcriptomes confirm these microbes within tumors, influencing local microenvironment and cytokine response. These microbes correlate significantly with the Epstein-Barr virus (EBV) loads in the nasopharynx, exhibiting an increased dose-response relationship. Collectively, our study identifies oral microbes migrating to the nasopharynx, infiltrating tumors, impacting microenvironments and linking with EBV infection. These results enhance our understanding of abnormal microbial communication and their roles in carcinogenesis. Oral microbes in non-oral locations are noted across various cancers. This study highlights the abnormal translocation of oral microbes to the nasopharynx, raising the risk of nasopharyngeal cancer by remodeling the tumor microenvironment and potentially influencing EBV infection.