Fast genetic mapping of complex traits in C. elegans using millions of individuals in bulk

Genetic studies of complex traits in animals have been hindered by the need to generate, maintain, and phenotype large panels of recombinant lines. We developed a new method, C. elegans eXtreme Quantitative Trait Locus ( ce X-QTL) mapping, that overcomes this obstacle via bulk selection on millions...

Full description

Saved in:
Bibliographic Details
Published in:Nature communications 2019-06, Vol.10 (1), p.2680-10, Article 2680
Main Authors: Burga, Alejandro, Ben-David, Eyal, Lemus Vergara, Tzitziki, Boocock, James, Kruglyak, Leonid
Format: Article
Language:English
Subjects:
45
631/208/207
631/208/480
Animals
Caenorhabditis elegans - genetics
Chromosome Mapping - economics
Chromosome Mapping - methods
Drug resistance
Drug Resistance - genetics
Female
Fitness
Gene expression
Gene Expression Regulation - genetics
Gene mapping
Genetic Fitness - genetics
Heat shock proteins
Humanities and Social Sciences
Male
Mapping
multidisciplinary
Mutation
Phenotypes
Quantitative trait loci
Quantitative Trait Loci - genetics
Quantitative Trait, Heritable
Reproductive fitness
Science
Science (multidisciplinary)
Time Factors
Transcription
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Genetic studies of complex traits in animals have been hindered by the need to generate, maintain, and phenotype large panels of recombinant lines. We developed a new method, C. elegans eXtreme Quantitative Trait Locus ( ce X-QTL) mapping, that overcomes this obstacle via bulk selection on millions of unique recombinant individuals. We use ce X-QTL to map a drug resistance locus with high resolution. We also map differences in gene expression in live worms and discovered that mutations in the co-chaperone sti-1 upregulate the transcription of HSP-90. Lastly, we use ce X-QTL to map loci that influence fitness genome-wide confirming previously reported causal variants and uncovering new fitness loci. ce X-QTL is fast, powerful and cost-effective, and will accelerate the study of complex traits in animals. QTL mapping in multicellular animals usually requires generation, maintenance, and phenotyping large panels of recombinant individuals. Here, the authors develop ce X-QTL mapping, a method for fast genetic mapping via bulk selection on millions of unique recombinant C. elegans individuals.
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-019-10636-9