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Theoretical and Experimental Studies on the Evidence of 1,3-β-Glucan in Marennine of Haslea ostrearia
Marennine, a blue pigment produced by the blue diatom , is known to have some biological activities. This pigment is responsible for the greening of oysters on the West Coast of France. Other new species of blue diatom, , sp. inedit., sp. inedit, and , also produce marennine-like pigments with simil...
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| Published in: | Molecules (Basel, Switzerland) Switzerland), 2023-07, Vol.28 (15), p.5625 |
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| creator | Yusuf, Muhammad Baroroh, Umi Nuwarda, Rina Fajri Prasetiya, Fiddy Semba Ishmayana, Safri Novianti, Mia Tria Tohari, Taufik Ramdani Hardianto, Ari Subroto, Toto Mouget, Jean-Luc Pasetto, Pamela |
| description | Marennine, a blue pigment produced by the blue diatom
, is known to have some biological activities. This pigment is responsible for the greening of oysters on the West Coast of France. Other new species of blue diatom,
,
sp. inedit.,
sp. inedit, and
, also produce marennine-like pigments with similar biological activities. Aside from being a potential source of natural blue pigments,
-like diatoms present a commercial potential for the aquaculture, food, cosmetics, and health industries. Unfortunately, for a hundred years, the exact molecular structure of this bioactive compound has remained a mystery. A lot of hypotheses regarding the chemical structure of marennine have been proposed. The recent discovery of this structure revealed that it is a macromolecule, mainly carbohydrates, with a complex composition. In this study, some glycoside hydrolases were used to digest marennine, and the products were further analyzed using nuclear magnetic resonance (NMR) and mass spectroscopy (MS). The reducing sugar assay showed that marennine was hydrolyzed only by endo-1,3-β-glucanase. Further insight into the structure of marennine was provided by the spectrum of
H NMR, MS, a colorimetric assay, and a computational study, which suggest that the chemical structure of marennine contains 1,3-β-glucan. |
| doi_str_mv | 10.3390/molecules28155625 |
| format | article |
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, is known to have some biological activities. This pigment is responsible for the greening of oysters on the West Coast of France. Other new species of blue diatom,
,
sp. inedit.,
sp. inedit, and
, also produce marennine-like pigments with similar biological activities. Aside from being a potential source of natural blue pigments,
-like diatoms present a commercial potential for the aquaculture, food, cosmetics, and health industries. Unfortunately, for a hundred years, the exact molecular structure of this bioactive compound has remained a mystery. A lot of hypotheses regarding the chemical structure of marennine have been proposed. The recent discovery of this structure revealed that it is a macromolecule, mainly carbohydrates, with a complex composition. In this study, some glycoside hydrolases were used to digest marennine, and the products were further analyzed using nuclear magnetic resonance (NMR) and mass spectroscopy (MS). The reducing sugar assay showed that marennine was hydrolyzed only by endo-1,3-β-glucanase. Further insight into the structure of marennine was provided by the spectrum of
H NMR, MS, a colorimetric assay, and a computational study, which suggest that the chemical structure of marennine contains 1,3-β-glucan.</description><identifier>ISSN: 1420-3049</identifier><identifier>EISSN: 1420-3049</identifier><identifier>DOI: 10.3390/molecules28155625</identifier><identifier>PMID: 37570595</identifier><language>eng</language><publisher>Switzerland: MDPI AG</publisher><subject>Bacteria ; Carbohydrates ; Cellulase ; computational study ; endo-1,3-β-glucanase ; Enzymes ; Glucose ; Haslea ostrearia ; hydrolysis ; Hypotheses ; marennine ; Molecular structure ; NMR ; Nuclear magnetic resonance ; Pigments ; β-glucan</subject><ispartof>Molecules (Basel, Switzerland), 2023-07, Vol.28 (15), p.5625</ispartof><rights>2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2023 by the authors. 2023</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c494t-898728f85a27dd50faf1e97218551bf169009ac3da084ae451e9b4dae6802b053</citedby><cites>FETCH-LOGICAL-c494t-898728f85a27dd50faf1e97218551bf169009ac3da084ae451e9b4dae6802b053</cites><orcidid>0000-0003-2011-7161 ; 0000-0002-1629-407X ; 0000-0001-6474-2319 ; 0000-0003-2209-8080 ; 0000-0001-6065-5437 ; 0000-0002-9825-4425 ; 0000-0003-1627-1553 ; 0000-0001-7868-0075</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2849096156/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2849096156?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/37570595$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Yusuf, Muhammad</creatorcontrib><creatorcontrib>Baroroh, Umi</creatorcontrib><creatorcontrib>Nuwarda, Rina Fajri</creatorcontrib><creatorcontrib>Prasetiya, Fiddy Semba</creatorcontrib><creatorcontrib>Ishmayana, Safri</creatorcontrib><creatorcontrib>Novianti, Mia Tria</creatorcontrib><creatorcontrib>Tohari, Taufik Ramdani</creatorcontrib><creatorcontrib>Hardianto, Ari</creatorcontrib><creatorcontrib>Subroto, Toto</creatorcontrib><creatorcontrib>Mouget, Jean-Luc</creatorcontrib><creatorcontrib>Pasetto, Pamela</creatorcontrib><title>Theoretical and Experimental Studies on the Evidence of 1,3-β-Glucan in Marennine of Haslea ostrearia</title><title>Molecules (Basel, Switzerland)</title><addtitle>Molecules</addtitle><description>Marennine, a blue pigment produced by the blue diatom
, is known to have some biological activities. This pigment is responsible for the greening of oysters on the West Coast of France. Other new species of blue diatom,
,
sp. inedit.,
sp. inedit, and
, also produce marennine-like pigments with similar biological activities. Aside from being a potential source of natural blue pigments,
-like diatoms present a commercial potential for the aquaculture, food, cosmetics, and health industries. Unfortunately, for a hundred years, the exact molecular structure of this bioactive compound has remained a mystery. A lot of hypotheses regarding the chemical structure of marennine have been proposed. The recent discovery of this structure revealed that it is a macromolecule, mainly carbohydrates, with a complex composition. In this study, some glycoside hydrolases were used to digest marennine, and the products were further analyzed using nuclear magnetic resonance (NMR) and mass spectroscopy (MS). The reducing sugar assay showed that marennine was hydrolyzed only by endo-1,3-β-glucanase. Further insight into the structure of marennine was provided by the spectrum of
H NMR, MS, a colorimetric assay, and a computational study, which suggest that the chemical structure of marennine contains 1,3-β-glucan.</description><subject>Bacteria</subject><subject>Carbohydrates</subject><subject>Cellulase</subject><subject>computational study</subject><subject>endo-1,3-β-glucanase</subject><subject>Enzymes</subject><subject>Glucose</subject><subject>Haslea ostrearia</subject><subject>hydrolysis</subject><subject>Hypotheses</subject><subject>marennine</subject><subject>Molecular structure</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Pigments</subject><subject>β-glucan</subject><issn>1420-3049</issn><issn>1420-3049</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2023</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNplksFu1DAQhiMEoqXwAFxQJC4cCHhiO7FPqKqWtlIRB8rZmjjjrldZe7GTCl6LB-GZMN1StXCyNfPPp_lHf1W9BPaOc83eb-NEdpkotwqk7Fr5qDoE0bKGM6Ef3_sfVM9y3jDWggD5tDrgveyZ1PKwcpdriolmb3GqMYz16vuOkt9SmEvhy7yMnnIdQz2vqV5d-5GCpTq6Gt7y5tfP5nRaLIbah_oTJgrBh5vuGeaJsI55ToTJ4_PqicMp04vb96j6-nF1eXLWXHw-PT85vmis0GJulFZ9q5yS2PbjKJlDB6T7FpSUMDjoNGMaLR-RKYEkZOkOYkTqFGsHJvlRdb7njhE3ZleMYPphInpzU4jpymAqZicyAzjOJOutc07IARBAOt2jGq0CxaGwPuxZu2XY0mjLSRJOD6APO8GvzVW8NsAEaCFFIby5JaT4baE8m63PlqYJA8Ulm1ZJxpnqeF-kr_-RbuKSQrlVUQnNdAeyKyrYq2yKOSdyd9sAM38iYf6LRJl5dd_G3cTfDPDfpfy0Uw</recordid><startdate>20230725</startdate><enddate>20230725</enddate><creator>Yusuf, Muhammad</creator><creator>Baroroh, Umi</creator><creator>Nuwarda, Rina Fajri</creator><creator>Prasetiya, Fiddy Semba</creator><creator>Ishmayana, Safri</creator><creator>Novianti, Mia Tria</creator><creator>Tohari, Taufik Ramdani</creator><creator>Hardianto, Ari</creator><creator>Subroto, Toto</creator><creator>Mouget, Jean-Luc</creator><creator>Pasetto, Pamela</creator><general>MDPI AG</general><general>MDPI</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>M1P</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0003-2011-7161</orcidid><orcidid>https://orcid.org/0000-0002-1629-407X</orcidid><orcidid>https://orcid.org/0000-0001-6474-2319</orcidid><orcidid>https://orcid.org/0000-0003-2209-8080</orcidid><orcidid>https://orcid.org/0000-0001-6065-5437</orcidid><orcidid>https://orcid.org/0000-0002-9825-4425</orcidid><orcidid>https://orcid.org/0000-0003-1627-1553</orcidid><orcidid>https://orcid.org/0000-0001-7868-0075</orcidid></search><sort><creationdate>20230725</creationdate><title>Theoretical and Experimental Studies on the Evidence of 1,3-β-Glucan in Marennine of Haslea ostrearia</title><author>Yusuf, Muhammad ; 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, is known to have some biological activities. This pigment is responsible for the greening of oysters on the West Coast of France. Other new species of blue diatom,
,
sp. inedit.,
sp. inedit, and
, also produce marennine-like pigments with similar biological activities. Aside from being a potential source of natural blue pigments,
-like diatoms present a commercial potential for the aquaculture, food, cosmetics, and health industries. Unfortunately, for a hundred years, the exact molecular structure of this bioactive compound has remained a mystery. A lot of hypotheses regarding the chemical structure of marennine have been proposed. The recent discovery of this structure revealed that it is a macromolecule, mainly carbohydrates, with a complex composition. In this study, some glycoside hydrolases were used to digest marennine, and the products were further analyzed using nuclear magnetic resonance (NMR) and mass spectroscopy (MS). The reducing sugar assay showed that marennine was hydrolyzed only by endo-1,3-β-glucanase. Further insight into the structure of marennine was provided by the spectrum of
H NMR, MS, a colorimetric assay, and a computational study, which suggest that the chemical structure of marennine contains 1,3-β-glucan.</abstract><cop>Switzerland</cop><pub>MDPI AG</pub><pmid>37570595</pmid><doi>10.3390/molecules28155625</doi><orcidid>https://orcid.org/0000-0003-2011-7161</orcidid><orcidid>https://orcid.org/0000-0002-1629-407X</orcidid><orcidid>https://orcid.org/0000-0001-6474-2319</orcidid><orcidid>https://orcid.org/0000-0003-2209-8080</orcidid><orcidid>https://orcid.org/0000-0001-6065-5437</orcidid><orcidid>https://orcid.org/0000-0002-9825-4425</orcidid><orcidid>https://orcid.org/0000-0003-1627-1553</orcidid><orcidid>https://orcid.org/0000-0001-7868-0075</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Open Access: PubMed Central; Publicly Available Content Database |
| subjects | Bacteria Carbohydrates Cellulase computational study endo-1,3-β-glucanase Enzymes Glucose Haslea ostrearia hydrolysis Hypotheses marennine Molecular structure NMR Nuclear magnetic resonance Pigments β-glucan |
| title | Theoretical and Experimental Studies on the Evidence of 1,3-β-Glucan in Marennine of Haslea ostrearia |
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