Biodegradation of the fungicide carbendazim by bacteria from Coriandrum sativum L. rhizosphere

• Published in: Acta scientiarum. Biological sciences 2017-01, Vol.39 (1), p.71
• Main Authors: Santos, Jucileuza Conceição dos, Batista, Ieda Hortêncio, Barroso, Hiléia Santos, Lima, João Marcelo Silva, Costa Neto, Pedro De Queiroz, Ghelfi, Andrea, Pereira, José Odair
• Format: Article
• Language: English
• Subjects: Amazon soil; Bacteria; Biodegradation; carbendazim-degrading bacteria; coriander; Farming; fungicide; Pesticides; Soil microorganisms; Stenotrophomonas; xenobiotics
• ISSN: 1679-9283; 1807-863X
• DOI: 10.4025/actascibiolsci.v39i1.34067

The biocidal agrochemicals commonly used in agriculture can remain in the soil, affecting the environmental conditions and causing serious risks to health. Knowing that soil microorganisms, especially those from the rhizosphere, can degrade environmental xenobiotics, it was evaluated the potential of bacteria isolated from Coriandrum sativum L. rhizosphere to biodegrade carbendazim (MBC), a fungicide extensively used by agriculturists from rural farming communities in Manaus, Amazonas. Cultures carried out in medium containing carbendazim as a sole carbon source enabled the isolation of 80 bacteria, in the established conditions. Assays to determine degradation potential allowed the selection of the two elite isolates identified as Stenotrophomonas sp. and Ochrobactrum sp. Quantitative assays with each strain individually or in consortium, were carried out using minimal salt medium added with carbendazim (250 µg mL-1) and incubated at 30°C, under agitation (125 rpm) for 21 days. Samples used in the biodegradation test were HPLC analyzed for final fungicide quantitation. The Stenotrophomonas sp. strain was more efficient (68.9%) to degrade carbendazim and showed no toxicity in tests with Artemia salina.