Exploring the Distinct Distribution of Archaeal Communities in Sites Contaminated with Explosives

Most of the research on bioremediation and estimation of microbial diversity in waste contaminated sites is focused on the domain , whereas details on the relevance of are still lacking. The present study examined the archaeal diversity and predicted metabolic pathways in two discrete sites (SITE1 a...

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Bibliographic Details
Published in:Biomolecules (Basel, Switzerland) Switzerland), 2022-03, Vol.12 (4), p.489
Main Authors: Pal, Yash, Mayilraj, Shanmugam, Krishnamurthi, Srinivasan
Format: Article
Language:English
Subjects:
amplicon sequencing
Archaea
Archaea - genetics
Bacteria
Biodegradation
Bioremediation
Candidate species
Ecosystem
Explosive Agents
Explosives
HMX (1,3, 5,7-Tetranitro-1,3,5,7-tetrazocane)
MEGAN
Metabolic pathways
Microbial contamination
Phylogeny
RDX (1,3,5-Trinitro-1,3,5-triazine)
RNA, Ribosomal, 16S - genetics
rRNA 16S
Sediments
Soil
Soil pollution
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Summary:Most of the research on bioremediation and estimation of microbial diversity in waste contaminated sites is focused on the domain , whereas details on the relevance of are still lacking. The present study examined the archaeal diversity and predicted metabolic pathways in two discrete sites (SITE1 and SITE2) contaminated with explosives (RDX and HMX) by amplicon-targeted sequencing of 16S rRNA genes. In total, 14 soil samples were processed, and 35,758 OTUs were observed, among which 981 OTUs were classified as , representing ~2.7% of the total microbial diversity in our samples. The majority of OTUs belonged to phyla (~49%), (~24%), and (~23%), while the remaining (~4%) OTUs were affiliated to Parvarchaeota, Aenigmarchaeota, and Diapherotrites. The comparative studies between explosives contaminated and agricultural soil samples (with no history of explosives contamination) displayed significant differences between the compositions of the archaeal communities. Further, the metabolic pathways pertaining to xenobiotic degradation were presumably more abundant in the contaminated sites. Our data provide a first comprehensive report of archaeal communities in explosives contaminated sites and their putative degradation role in such ecosystems which have been as yet unexplored.
ISSN:2218-273X
2218-273X
DOI:10.3390/biom12040489