Defined Pig Microbiota with a Potential Protective Effect against Infection with Salmonella Typhimurium

A balanced microbiota is a main prerequisite for the host's health. The aim of the present work was to develop defined pig microbiota (DPM) with the potential ability to protect piglets against infection with Typhimurium, which causes enterocolitis. A total of 284 bacterial strains were isolate...

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Bibliographic Details
Published in:Microorganisms (Basel) 2023-04, Vol.11 (4), p.1007
Main Authors: Horvathova, Kristyna, Modrackova, Nikol, Splichal, Igor, Splichalova, Alla, Amin, Ahmad, Ingribelli, Eugenio, Killer, Jiri, Doskocil, Ivo, Pechar, Radko, Kodesova, Tereza, Vlkova, Eva
Format: Article
Language:English
Subjects:
Animals
Antibiotics
Bacteria
bacterial consortium
Control
Culture media
Domestic animals
Enterocolitis
Epithelial cells
Epithelium
Feces
Freezing
Gastroenteritis
Genera
Genetic aspects
Glycerol
gnotobiotic piglets
Health aspects
Hogs
Identification and classification
Infections
Infectious diseases
intestinal pathogens
Limosilactobacillus reuteri
Mass spectrometry
Mass spectroscopy
Microbiota
Microbiota (Symbiotic organisms)
Microorganisms
Pathogens
Phenotypes
Physiological aspects
pig intestinal bacteria
probiotic properties testing
Probiotics
rRNA 16S
Salmonella
Salmonella Typhimurium
Selective media
Strains (organisms)
Swine
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Summary:A balanced microbiota is a main prerequisite for the host's health. The aim of the present work was to develop defined pig microbiota (DPM) with the potential ability to protect piglets against infection with Typhimurium, which causes enterocolitis. A total of 284 bacterial strains were isolated from the colon and fecal samples of wild and domestic pigs or piglets using selective and nonselective cultivation media. Isolates belonging to 47 species from 11 different genera were identified by MALDI-TOF mass spectrometry (MALDI-TOF MS). The bacterial strains for the DPM were selected for anti- activity, ability to aggregate, adherence to epithelial cells, and to be bile and acid tolerant. The selected combination of 9 strains was identified by sequencing of the 16S rRNA gene as sp., subsp. , , , , subsp. , subsp. , and (two strains) did not show mutual inhibition, and the mixture was stable under freezing for at least 6 months. Moreover, strains were classified as safe without pathogenic phenotype and resistance to antibiotics. Future experiments with -infected piglets are needed to test the protective effect of the developed DPM.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms11041007