Diversity, Phylogeny and Plant Growth Promotion Traits of Nodule Associated Bacteria Isolated from Lotus parviflorus

spp. are widely used as a forage to improve pastures, and inoculation with elite rhizobial strains is a common practice in many countries. However, only a few species have been studied in the context of plant-rhizobia interactions. In this study, forty highly diverse bacterial strains were isolated...

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Bibliographic Details
Published in:Microorganisms (Basel) 2020-03, Vol.8 (4), p.499
Main Authors: Soares, Ricardo, Trejo, Jesús, Lorite, Maria J, Figueira, Etelvina, Sanjuán, Juan, Videira E Castro, Isabel
Format: Article
Language:English
Subjects:
Automation
Bacteria
Bradyrhizobium
Deoxyribonucleic acid
diversity
DNA
endophytes
Gene amplification
Genera
Host range
Inoculation
Legumes
Leguminous plants
Lotus parviflorus
Lytic enzymes
Methods
NifH gene
Nitrogen
Nodules
Pasture
Phylogenetics
Phylogeny
Plant growth
plant growth promoting rhizobacteria (PGPR)
Promotion
rhizobia
Root nodules
Solubilization
Strains (organisms)
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Summary:spp. are widely used as a forage to improve pastures, and inoculation with elite rhizobial strains is a common practice in many countries. However, only a few species have been studied in the context of plant-rhizobia interactions. In this study, forty highly diverse bacterial strains were isolated from root nodules of wild plants growing in two field locations in Portugal. However, only 10% of these isolates could nodulate one or more legume hosts tested, whereas 90% were thought to be opportunistic nodule associated bacteria. Phylogenetic studies place the nodulating isolates within the genus, which is closely related to and other sp. strains isolated from genistoid legumes and spp. Symbiotic and gene phylogenies were fully consistent with the taxonomic assignment and host range. The non-nodulating bacteria isolated were alpha- ( ), beta- ( ) and gamma-proteobacteria ( , , and ), as well as some bacteroidetes from genera and r. Some of these nodule-associated bacteria expressed plant growth promotion (PGP) traits, such as production of lytic enzymes, antagonistic activity against phytopathogens, phosphate solubilization, or siderophore production. This argues for a potential beneficial role of these nodule-associated bacteria.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms8040499