Ex Situ and In Situ Artificial Thermo-Aging Study of the Natural Degradation of Bombyx mori Silk Fibroin

This study investigates the degradation mechanism of silk fibroin through Fourier-transformed infrared spectroscopy (FTIR) analysis. The secondary structure of silk fibroin-based materials is monitored using FTIR, and various estimators are calculated to assess the impact of degradation conditions a...

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Bibliographic Details
Published in:Applied sciences 2023-08, Vol.13 (16), p.9427
Main Authors: Koperska, Monika A., Bagniuk, Jacek, Zaitz-Olsza, Małgorzata M., Gassowska, Katarzyna, Pawcenis, Dominika, Sitarz, Maciej, Bulska, Ewa, Profic-Paczkowska, Joanna
Format: Article
Language:English
Subjects:
16th century
Aging
Amino acids
artificial aging
crystallinity estimators
Experiments
fibroin
in situ ATR FTIR
Museums
Oxidation
pol-ATR
silk
Spectrum analysis
Temperature
Trends
VOCs
Volatile organic compounds
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Summary:This study investigates the degradation mechanism of silk fibroin through Fourier-transformed infrared spectroscopy (FTIR) analysis. The secondary structure of silk fibroin-based materials is monitored using FTIR, and various estimators are calculated to assess the impact of degradation conditions and aging time. The oxidation estimator shows consistent growth, indicating peptide bond oxidation from the early stages of artificial aging, regardless of the conditions. The environment influences the hydrolysis estimator, with water introduction leading to significant changes. The crystallinity estimator reflects the overall degradation level, affected by oxidation and hydrolysis. XRD and FTIR analysis of historical silk banners up to 500 years old demonstrate a decrease in crystallinity and an increase in hydrolysis and oxidation. The presence of water accelerates the oxidation process, while crystallinity changes are primarily driven by oxidation. Fibroin degradation affects both antiparallel and parallel regions, with water playing a crucial role in accelerating hydrolysis and causing structural shifts. This study enhances our understanding of silk fibroin degradation and provides valuable insights for preserving historical silk artifacts.
ISSN:2076-3417
2076-3417
DOI:10.3390/app13169427