Structural and functional fine mapping of cysteines in mammalian glutaredoxin reveal their differential oxidation susceptibility

• Published in: Nature communications 2023-07, Vol.14 (1), p.4550-4550, Article 4550
• Main Authors: Corteselli, Elizabeth M., Sharafi, Mona, Hondal, Robert, MacPherson, Maximilian, White, Sheryl, Lam, Ying-Wai, Gold, Clarissa, Manuel, Allison M., van der Vliet, Albert, Schneebeli, Severin T., Anathy, Vikas, Li, Jianing, Janssen-Heininger, Yvonne M. W.
• Format: Article
• Language: English
• Subjects: 101/58; 119/118; 631/45/607/1168; 631/535/1267; 82/80; Animals; Cellular structure; Combinatorial analysis; Conjugation; Cysteine - metabolism; Deactivation; Glutaredoxin; Glutaredoxins - genetics; Glutaredoxins - metabolism; Glutathione; Glutathione - metabolism; Humanities and Social Sciences; Hydrogen peroxide; Inactivation; Mammals - metabolism; Modelling; Molecular modelling; multidisciplinary; Oxidation; Oxidation resistance; Oxidation-Reduction; Peptide mapping; Post-translation; Protein thiols; Proteins; Proteins - metabolism; Science; Science (multidisciplinary); Site-directed mutagenesis; Structure-function relationships; Thiols
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-023-39664-2

Protein-S-glutathionylation is a post-translational modification involving the conjugation of glutathione to protein thiols, which can modulate the activity and structure of key cellular proteins. Glutaredoxins (GLRX) are oxidoreductases that regulate this process by performing deglutathionylation. However, GLRX has five cysteines that are potentially vulnerable to oxidative modification, which is associated with GLRX aggregation and loss of activity. To date, GLRX cysteines that are oxidatively modified and their relative susceptibilities remain unknown. We utilized molecular modeling approaches, activity assays using recombinant GLRX, coupled with site-directed mutagenesis of each cysteine both individually and in combination to address the oxidizibility of GLRX cysteines. These approaches reveal that C8 and C83 are targets for S-glutathionylation and oxidation by hydrogen peroxide in vitro. In silico modeling and experimental validation confirm a prominent role of C8 for dimer formation and aggregation. Lastly, combinatorial mutation of C8, C26, and C83 results in increased activity of GLRX and resistance to oxidative inactivation and aggregation. Results from these integrated computational and experimental studies provide insights into the relative oxidizability of GLRX’s cysteines and have implications for the use of GLRX as a therapeutic in settings of dysregulated protein glutathionylation. Glutaredoxin (GLRX) is a key enzyme in redox regulation via oxidation of protein cysteines and its activity is disrupted in diverse human diseases. This study integrates molecular modeling and biochemical validation to provide insights into the mechanisms of oxidative inactivation of GLRX.