Genomic sequencing of two isolates of Ralstonia solanacearum causing Sergipe facies and comparative analysis with Bugtok disease isolates

Ralstonia solanacearum is the causal agent of Moko disease in bananas, which in the state of Sergipe in northeastern Brazil causes "Sergipe facies". This disease induces atypical symptoms similar to those of Bugtok disease in the Philippines. This study was conducted to sequence, assemble,...

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Bibliographic Details
Published in:Genetics and molecular biology 2020-01, Vol.43 (4), p.e20200155-e20200155
Main Authors: Silva, Jéssica Rodrigues da, Pais, Ana Karolina Leite, Albuquerque, Greecy Mirian Rodrigues, Silva, Adriano Márcio Freire, Silva Junior, Wilson José, Balbino, Valdir de Queiroz, Fonseca, Maria Esther Noronha, Gama, Marco Aurélio Siqueira da, Souza, Elineide Barbosa de, Mariano, Rosa de Lima Ramos
Format: Article
Language:English
Subjects:
Banana tree
BIOCHEMISTRY & MOLECULAR BIOLOGY
GENETICS & HEREDITY
Genetics of Microorganisms
inflorescence infection
phylotype
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Summary:Ralstonia solanacearum is the causal agent of Moko disease in bananas, which in the state of Sergipe in northeastern Brazil causes "Sergipe facies". This disease induces atypical symptoms similar to those of Bugtok disease in the Philippines. This study was conducted to sequence, assemble, and annotate the genomes of the Sergipe facies-causing isolates SFC and IBSBF2570 (sequevar IIA-53) and compare their genomes with two representative isolates causing Bugtok disease. The genomes were sequenced and assembled, resulting in lengths of 5.58 Mb (SFC) and 5.46 Mb (IBSBF2570) in 185 and 174 contigs, respectively. The isolates of Sergipe facies and Bugtok disease showed similarities in their gene contents. We identified 5,668 information clusters, 3,752 of which were shared by all genomes (core genes). Moreover, 3,585 single-copy genes were identified. Isolates causing Bugtok disease exclusively shared 266 more information clusters than the isolates causing Sergipe facies. These results suggest that Sergipe facies and Bugtok disease isolates show high genomic similarity. However, the similarity is even greater between the Bugtok disease isolates. This may be because of their longer period of interaction compared to Sergipe facies isolates.
ISSN:1415-4757
1678-4685
1678-4685
DOI:10.1590/1678-4685-GMB-2020-0155