Elevated Pentraxin 3 in Obese Adipose Tissue Promotes Adipogenic Differentiation by Activating Neuropeptide Y Signaling

Obesity is accompanied by chronic systemic inflammation characterized by macrophage infiltration of obese tissues, an elevated plasma level of inflammatory substances, and excessive accumulation of lipids. The pro-inflammatory factor pentraxin 3 (PTX3) is also elevated in obese tissues, suggesting i...

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Bibliographic Details
Published in:Frontiers in immunology 2018-07, Vol.9, p.1790-1790
Main Authors: Shin, Min-Kyung, Choi, Bongkun, Kim, Eun-Young, Park, Ji-Eun, Hwang, Eui Seung, Lee, Hyang Ju, Kim, Min Kyung, Kim, Ji-Eun, Kim, Seong Who, Chang, Eun-Ju
Format: Article
Language:English
Subjects:
adipogenesis
Immunology
macrophage
neuropeptide Y
obesity
pentraxin3
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Summary:Obesity is accompanied by chronic systemic inflammation characterized by macrophage infiltration of obese tissues, an elevated plasma level of inflammatory substances, and excessive accumulation of lipids. The pro-inflammatory factor pentraxin 3 (PTX3) is also elevated in obese tissues, suggesting its potential role in adipogenesis. We found by analyzing murine preadipocyte 3T3-L1 cells, and human adipocytes derived from mesenchymal stem cells, which locally elevated PTX3 in obese adipose tissue augments adipocyte differentiation and subsequent lipid accumulation. This occurs the upregulation of adipogenesis-related transcription factors. PTX3 enhanced lipid accumulation in murine 3T3-L1 cells by upregulating the expression of neuropeptide Y (NPY)/NPY receptor (NPYR) expression in preadipocytes. Pharmacological inhibition by NPYR antagonists abolished these effects. NPY also promoted the production of reactive oxygen species (ROS), a known trigger of adipogenesis. NPYR antagonists as well as antioxidant -acetylcysteine showed anti-adipogenic effects by reducing the ROS levels, indicating that PTX3 mediates adipogenesis through NPY-dependent ROS production. These findings suggest that PTX3 plays a key role in the development of obesity by enhancing adipocyte differentiation and lipid synthesis NPY/NPYR signaling. These observations provide a mechanistic explanation for the adipogenesis mediated by PTX3.
ISSN:1664-3224
1664-3224
DOI:10.3389/fimmu.2018.01790