Intranasally Administered Extracellular Vesicles from Adipose Stem Cells Have Immunomodulatory Effects in a Mouse Model of Asthma

Asthma is a chronic eosinophilic airway disease characterized by type 2 helper T cell-driven inflammation. Adipose stem cells (ASCs) and the ASC culture supernatant are known to improve allergic airway inflammation; however, the immunomodulatory effects of ASC-derived extracellular vesicles (EVs) on...

Full description

Saved in:
Bibliographic Details
Published in:Stem cells international 2021-07, Vol.2021, p.6686625-11
Main Authors: Mun, Sue Jean, Kang, Shin Ae, Park, Hye-Kyung, Yu, Hak Sun, Cho, Kyu-Sup, Roh, Hwan-Jung
Format: Article
Language:English
Subjects:
Albumin
Allergens
Allergic diseases
Allergies
Analysis
Asthma
Body fat
Bronchus
Cell culture
Cytokines
Eosinophils
Extracellular vesicles
Immunoglobulin G
Immunoglobulins
Immunomodulation
Inflammation
Interleukins
Lavage
Leukocytes (eosinophilic)
Lungs
Lymph nodes
Lymphatic system
Lymphocytes
Lymphocytes T
Mice
Ovalbumin
Respiratory tract diseases
Stem cell transplantation
Stem cells
T cells
Vesicles
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c612t-fd0e1f0d90b0a40c212aa0ffe684c97cf1fad7ea4558cbb161e0bd21dcce55bb3
cites cdi_FETCH-LOGICAL-c612t-fd0e1f0d90b0a40c212aa0ffe684c97cf1fad7ea4558cbb161e0bd21dcce55bb3
container_end_page 11
container_issue
container_start_page 6686625
container_title Stem cells international
container_volume 2021
creator Mun, Sue Jean
Kang, Shin Ae
Park, Hye-Kyung
Yu, Hak Sun
Cho, Kyu-Sup
Roh, Hwan-Jung
description Asthma is a chronic eosinophilic airway disease characterized by type 2 helper T cell-driven inflammation. Adipose stem cells (ASCs) and the ASC culture supernatant are known to improve allergic airway inflammation; however, the immunomodulatory effects of ASC-derived extracellular vesicles (EVs) on allergic airway diseases remain unclear. Thus, we assessed the effects of ASC-derived EVs on allergic airway inflammation in a mouse model of asthma. EVs were isolated from the culture supernatant of murine ASCs and characterized. Six-week-old female C57BL/6 mice were sensitized to ovalbumin (OVA) by intraperitoneal injection and challenged intranasally with OVA. Before the OVA challenge, 10 μg/50 μl of ASC-derived EVs was administered intranasally to the experimental group. ASC-derived EVs significantly attenuated airway hyperresponsiveness (AHR) in asthmatic mice (p=0.023). ASC-derived EVs resulted in a remarkable reduction of the total number of inflammatory cells (p=0.005) and eosinophils (p=0.023) in the bronchoalveolar lavage fluid (BALF), the degree of eosinophilic lung inflammation (p
doi_str_mv 10.1155/2021/6686625
format article
fullrecord <record><control><sourceid>gale_doaj_</sourceid><recordid>TN_cdi_doaj_primary_oai_doaj_org_article_b85443e15a274f88ab34266920ee2fce</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A696974836</galeid><doaj_id>oai_doaj_org_article_b85443e15a274f88ab34266920ee2fce</doaj_id><sourcerecordid>A696974836</sourcerecordid><originalsourceid>FETCH-LOGICAL-c612t-fd0e1f0d90b0a40c212aa0ffe684c97cf1fad7ea4558cbb161e0bd21dcce55bb3</originalsourceid><addsrcrecordid>eNp9ks2LEzEYhwdR3KXuzbMEBBG0u0lmJjNzEUqpbmEXD37gLWSSN23KJKnJzGqP_udmbK1bEZNDQvK8D-SXN8ueEnxJSFleUUzJFWM1Y7R8kJ0TVlfThlX1w-OefTnLLmLc4DTyBheYPs7O8qJumobi8-zH0vVBOBFF1-3QTFnjTOwhgEKL7-lGQtcNnQjoM0QjO4hIB28TaLY-AvrQg0XzxER0Le4ALa0dnLdepZrehx1aaA2yj8g4JNCtH1LNrVfQIa_RLPZrK55kj7ToIlwc1kn26e3i4_x6evP-3XI-u5lKRmg_1QoD0Vg1uMWiwJISKgROdlYXsqmkJlqoCkRRlrVsW8II4FZRoqSEsmzbfJIt917lxYZvg7Ei7LgXhv868GHFRejHN_K2LosiB1IKWhW6rkWbF5SxFBgA1RKS683etR1aC0rCGGJ3Ij29cWbNV_6Op48qRvcke3kQBP91gNhza-KYtXCQQuKUEYyrOmcj-vwvdOOH4FJUicINKfOc5n-olUgPME778fNGKZ-xhjVVkWSJuvwHlaYCa6R3oE06Pyl4ca9gDaLr19F3Q2-8i6fg6z0og48xgD6GQTAfW5WPrcoPrZrwZ_cDPMK_GzMBr_bA2jglvpn_634Cxqvw7Q</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>2609153323</pqid></control><display><type>article</type><title>Intranasally Administered Extracellular Vesicles from Adipose Stem Cells Have Immunomodulatory Effects in a Mouse Model of Asthma</title><source>Wiley Online Library Open Access</source><source>Publicly Available Content Database</source><source>PubMed Central (PMC)</source><creator>Mun, Sue Jean ; Kang, Shin Ae ; Park, Hye-Kyung ; Yu, Hak Sun ; Cho, Kyu-Sup ; Roh, Hwan-Jung</creator><contributor>Abdelalim, Essam M. ; Essam M Abdelalim</contributor><creatorcontrib>Mun, Sue Jean ; Kang, Shin Ae ; Park, Hye-Kyung ; Yu, Hak Sun ; Cho, Kyu-Sup ; Roh, Hwan-Jung ; Abdelalim, Essam M. ; Essam M Abdelalim</creatorcontrib><description>Asthma is a chronic eosinophilic airway disease characterized by type 2 helper T cell-driven inflammation. Adipose stem cells (ASCs) and the ASC culture supernatant are known to improve allergic airway inflammation; however, the immunomodulatory effects of ASC-derived extracellular vesicles (EVs) on allergic airway diseases remain unclear. Thus, we assessed the effects of ASC-derived EVs on allergic airway inflammation in a mouse model of asthma. EVs were isolated from the culture supernatant of murine ASCs and characterized. Six-week-old female C57BL/6 mice were sensitized to ovalbumin (OVA) by intraperitoneal injection and challenged intranasally with OVA. Before the OVA challenge, 10 μg/50 μl of ASC-derived EVs was administered intranasally to the experimental group. ASC-derived EVs significantly attenuated airway hyperresponsiveness (AHR) in asthmatic mice (p=0.023). ASC-derived EVs resulted in a remarkable reduction of the total number of inflammatory cells (p=0.005) and eosinophils (p=0.023) in the bronchoalveolar lavage fluid (BALF), the degree of eosinophilic lung inflammation (p&lt;0.001), and the serum total and OVA-specific immunoglobulin (Ig)E (p=0.048 and p=0.001) and total IgG1 (p&lt;0.001). Interleukin- (IL-) 4 was significantly inhibited with ASC-derived EV pretreatment in the BALF and lung draining lymph nodes (LLNs) (p=0.040 and p=0.011). Furthermore, ASC-derived EV administration resulted in a significant increase of the regulatory T cell (Treg) populations in LLNs. ASC-derived EVs alleviated AHR and allergic airway inflammation caused by the induction of Treg expansion in a mouse model of asthma. There seems to be a role for ASC-derived EVs as a modifier in allergic airway disease.</description><identifier>ISSN: 1687-966X</identifier><identifier>ISSN: 1687-9678</identifier><identifier>EISSN: 1687-9678</identifier><identifier>DOI: 10.1155/2021/6686625</identifier><identifier>PMID: 34899920</identifier><language>eng</language><publisher>United States: Hindawi</publisher><subject>Albumin ; Allergens ; Allergic diseases ; Allergies ; Analysis ; Asthma ; Body fat ; Bronchus ; Cell culture ; Cytokines ; Eosinophils ; Extracellular vesicles ; Immunoglobulin G ; Immunoglobulins ; Immunomodulation ; Inflammation ; Interleukins ; Lavage ; Leukocytes (eosinophilic) ; Lungs ; Lymph nodes ; Lymphatic system ; Lymphocytes ; Lymphocytes T ; Mice ; Ovalbumin ; Respiratory tract diseases ; Stem cell transplantation ; Stem cells ; T cells ; Vesicles</subject><ispartof>Stem cells international, 2021-07, Vol.2021, p.6686625-11</ispartof><rights>Copyright © 2021 Sue Jean Mun et al.</rights><rights>COPYRIGHT 2021 John Wiley &amp; Sons, Inc.</rights><rights>Copyright © 2021 Sue Jean Mun et al. This is an open access article distributed under the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. https://creativecommons.org/licenses/by/4.0</rights><rights>Copyright © 2021 Sue Jean Mun et al. 2021</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c612t-fd0e1f0d90b0a40c212aa0ffe684c97cf1fad7ea4558cbb161e0bd21dcce55bb3</citedby><cites>FETCH-LOGICAL-c612t-fd0e1f0d90b0a40c212aa0ffe684c97cf1fad7ea4558cbb161e0bd21dcce55bb3</cites><orcidid>0000-0002-3055-8713 ; 0000-0002-4381-6996 ; 0000-0003-4486-7178</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.proquest.com/docview/2609153323/fulltextPDF?pq-origsite=primo$$EPDF$$P50$$Gproquest$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2609153323?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,885,25753,27924,27925,37012,37013,44590,53791,53793,75126</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/34899920$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Abdelalim, Essam M.</contributor><contributor>Essam M Abdelalim</contributor><creatorcontrib>Mun, Sue Jean</creatorcontrib><creatorcontrib>Kang, Shin Ae</creatorcontrib><creatorcontrib>Park, Hye-Kyung</creatorcontrib><creatorcontrib>Yu, Hak Sun</creatorcontrib><creatorcontrib>Cho, Kyu-Sup</creatorcontrib><creatorcontrib>Roh, Hwan-Jung</creatorcontrib><title>Intranasally Administered Extracellular Vesicles from Adipose Stem Cells Have Immunomodulatory Effects in a Mouse Model of Asthma</title><title>Stem cells international</title><addtitle>Stem Cells Int</addtitle><description>Asthma is a chronic eosinophilic airway disease characterized by type 2 helper T cell-driven inflammation. Adipose stem cells (ASCs) and the ASC culture supernatant are known to improve allergic airway inflammation; however, the immunomodulatory effects of ASC-derived extracellular vesicles (EVs) on allergic airway diseases remain unclear. Thus, we assessed the effects of ASC-derived EVs on allergic airway inflammation in a mouse model of asthma. EVs were isolated from the culture supernatant of murine ASCs and characterized. Six-week-old female C57BL/6 mice were sensitized to ovalbumin (OVA) by intraperitoneal injection and challenged intranasally with OVA. Before the OVA challenge, 10 μg/50 μl of ASC-derived EVs was administered intranasally to the experimental group. ASC-derived EVs significantly attenuated airway hyperresponsiveness (AHR) in asthmatic mice (p=0.023). ASC-derived EVs resulted in a remarkable reduction of the total number of inflammatory cells (p=0.005) and eosinophils (p=0.023) in the bronchoalveolar lavage fluid (BALF), the degree of eosinophilic lung inflammation (p&lt;0.001), and the serum total and OVA-specific immunoglobulin (Ig)E (p=0.048 and p=0.001) and total IgG1 (p&lt;0.001). Interleukin- (IL-) 4 was significantly inhibited with ASC-derived EV pretreatment in the BALF and lung draining lymph nodes (LLNs) (p=0.040 and p=0.011). Furthermore, ASC-derived EV administration resulted in a significant increase of the regulatory T cell (Treg) populations in LLNs. ASC-derived EVs alleviated AHR and allergic airway inflammation caused by the induction of Treg expansion in a mouse model of asthma. There seems to be a role for ASC-derived EVs as a modifier in allergic airway disease.</description><subject>Albumin</subject><subject>Allergens</subject><subject>Allergic diseases</subject><subject>Allergies</subject><subject>Analysis</subject><subject>Asthma</subject><subject>Body fat</subject><subject>Bronchus</subject><subject>Cell culture</subject><subject>Cytokines</subject><subject>Eosinophils</subject><subject>Extracellular vesicles</subject><subject>Immunoglobulin G</subject><subject>Immunoglobulins</subject><subject>Immunomodulation</subject><subject>Inflammation</subject><subject>Interleukins</subject><subject>Lavage</subject><subject>Leukocytes (eosinophilic)</subject><subject>Lungs</subject><subject>Lymph nodes</subject><subject>Lymphatic system</subject><subject>Lymphocytes</subject><subject>Lymphocytes T</subject><subject>Mice</subject><subject>Ovalbumin</subject><subject>Respiratory tract diseases</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>T cells</subject><subject>Vesicles</subject><issn>1687-966X</issn><issn>1687-9678</issn><issn>1687-9678</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNp9ks2LEzEYhwdR3KXuzbMEBBG0u0lmJjNzEUqpbmEXD37gLWSSN23KJKnJzGqP_udmbK1bEZNDQvK8D-SXN8ueEnxJSFleUUzJFWM1Y7R8kJ0TVlfThlX1w-OefTnLLmLc4DTyBheYPs7O8qJumobi8-zH0vVBOBFF1-3QTFnjTOwhgEKL7-lGQtcNnQjoM0QjO4hIB28TaLY-AvrQg0XzxER0Le4ALa0dnLdepZrehx1aaA2yj8g4JNCtH1LNrVfQIa_RLPZrK55kj7ToIlwc1kn26e3i4_x6evP-3XI-u5lKRmg_1QoD0Vg1uMWiwJISKgROdlYXsqmkJlqoCkRRlrVsW8II4FZRoqSEsmzbfJIt917lxYZvg7Ei7LgXhv868GHFRejHN_K2LosiB1IKWhW6rkWbF5SxFBgA1RKS683etR1aC0rCGGJ3Ij29cWbNV_6Op48qRvcke3kQBP91gNhza-KYtXCQQuKUEYyrOmcj-vwvdOOH4FJUicINKfOc5n-olUgPME778fNGKZ-xhjVVkWSJuvwHlaYCa6R3oE06Pyl4ca9gDaLr19F3Q2-8i6fg6z0og48xgD6GQTAfW5WPrcoPrZrwZ_cDPMK_GzMBr_bA2jglvpn_634Cxqvw7Q</recordid><startdate>20210731</startdate><enddate>20210731</enddate><creator>Mun, Sue Jean</creator><creator>Kang, Shin Ae</creator><creator>Park, Hye-Kyung</creator><creator>Yu, Hak Sun</creator><creator>Cho, Kyu-Sup</creator><creator>Roh, Hwan-Jung</creator><general>Hindawi</general><general>John Wiley &amp; Sons, Inc</general><general>Hindawi Limited</general><scope>RHU</scope><scope>RHW</scope><scope>RHX</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QO</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7X7</scope><scope>7XB</scope><scope>8AO</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>8G5</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>GUQSH</scope><scope>H94</scope><scope>K9.</scope><scope>M0S</scope><scope>M2O</scope><scope>MBDVC</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>Q9U</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-3055-8713</orcidid><orcidid>https://orcid.org/0000-0002-4381-6996</orcidid><orcidid>https://orcid.org/0000-0003-4486-7178</orcidid></search><sort><creationdate>20210731</creationdate><title>Intranasally Administered Extracellular Vesicles from Adipose Stem Cells Have Immunomodulatory Effects in a Mouse Model of Asthma</title><author>Mun, Sue Jean ; Kang, Shin Ae ; Park, Hye-Kyung ; Yu, Hak Sun ; Cho, Kyu-Sup ; Roh, Hwan-Jung</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c612t-fd0e1f0d90b0a40c212aa0ffe684c97cf1fad7ea4558cbb161e0bd21dcce55bb3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Albumin</topic><topic>Allergens</topic><topic>Allergic diseases</topic><topic>Allergies</topic><topic>Analysis</topic><topic>Asthma</topic><topic>Body fat</topic><topic>Bronchus</topic><topic>Cell culture</topic><topic>Cytokines</topic><topic>Eosinophils</topic><topic>Extracellular vesicles</topic><topic>Immunoglobulin G</topic><topic>Immunoglobulins</topic><topic>Immunomodulation</topic><topic>Inflammation</topic><topic>Interleukins</topic><topic>Lavage</topic><topic>Leukocytes (eosinophilic)</topic><topic>Lungs</topic><topic>Lymph nodes</topic><topic>Lymphatic system</topic><topic>Lymphocytes</topic><topic>Lymphocytes T</topic><topic>Mice</topic><topic>Ovalbumin</topic><topic>Respiratory tract diseases</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>T cells</topic><topic>Vesicles</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mun, Sue Jean</creatorcontrib><creatorcontrib>Kang, Shin Ae</creatorcontrib><creatorcontrib>Park, Hye-Kyung</creatorcontrib><creatorcontrib>Yu, Hak Sun</creatorcontrib><creatorcontrib>Cho, Kyu-Sup</creatorcontrib><creatorcontrib>Roh, Hwan-Jung</creatorcontrib><collection>Hindawi Publishing Complete</collection><collection>Hindawi Publishing Subscription Journals</collection><collection>Hindawi Publishing Open Access</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Biotechnology Research Abstracts</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Health &amp; Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>ProQuest Pharma Collection</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Research Library (Alumni Edition)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central</collection><collection>ProQuest Central Essentials</collection><collection>AUTh Library subscriptions: ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>Research Library Prep</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>ProQuest Health &amp; Medical Complete (Alumni)</collection><collection>Health &amp; Medical Collection (Alumni Edition)</collection><collection>ProQuest research library</collection><collection>Research Library (Corporate)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central Basic</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Stem cells international</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mun, Sue Jean</au><au>Kang, Shin Ae</au><au>Park, Hye-Kyung</au><au>Yu, Hak Sun</au><au>Cho, Kyu-Sup</au><au>Roh, Hwan-Jung</au><au>Abdelalim, Essam M.</au><au>Essam M Abdelalim</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Intranasally Administered Extracellular Vesicles from Adipose Stem Cells Have Immunomodulatory Effects in a Mouse Model of Asthma</atitle><jtitle>Stem cells international</jtitle><addtitle>Stem Cells Int</addtitle><date>2021-07-31</date><risdate>2021</risdate><volume>2021</volume><spage>6686625</spage><epage>11</epage><pages>6686625-11</pages><issn>1687-966X</issn><issn>1687-9678</issn><eissn>1687-9678</eissn><abstract>Asthma is a chronic eosinophilic airway disease characterized by type 2 helper T cell-driven inflammation. Adipose stem cells (ASCs) and the ASC culture supernatant are known to improve allergic airway inflammation; however, the immunomodulatory effects of ASC-derived extracellular vesicles (EVs) on allergic airway diseases remain unclear. Thus, we assessed the effects of ASC-derived EVs on allergic airway inflammation in a mouse model of asthma. EVs were isolated from the culture supernatant of murine ASCs and characterized. Six-week-old female C57BL/6 mice were sensitized to ovalbumin (OVA) by intraperitoneal injection and challenged intranasally with OVA. Before the OVA challenge, 10 μg/50 μl of ASC-derived EVs was administered intranasally to the experimental group. ASC-derived EVs significantly attenuated airway hyperresponsiveness (AHR) in asthmatic mice (p=0.023). ASC-derived EVs resulted in a remarkable reduction of the total number of inflammatory cells (p=0.005) and eosinophils (p=0.023) in the bronchoalveolar lavage fluid (BALF), the degree of eosinophilic lung inflammation (p&lt;0.001), and the serum total and OVA-specific immunoglobulin (Ig)E (p=0.048 and p=0.001) and total IgG1 (p&lt;0.001). Interleukin- (IL-) 4 was significantly inhibited with ASC-derived EV pretreatment in the BALF and lung draining lymph nodes (LLNs) (p=0.040 and p=0.011). Furthermore, ASC-derived EV administration resulted in a significant increase of the regulatory T cell (Treg) populations in LLNs. ASC-derived EVs alleviated AHR and allergic airway inflammation caused by the induction of Treg expansion in a mouse model of asthma. There seems to be a role for ASC-derived EVs as a modifier in allergic airway disease.</abstract><cop>United States</cop><pub>Hindawi</pub><pmid>34899920</pmid><doi>10.1155/2021/6686625</doi><tpages>11</tpages><orcidid>https://orcid.org/0000-0002-3055-8713</orcidid><orcidid>https://orcid.org/0000-0002-4381-6996</orcidid><orcidid>https://orcid.org/0000-0003-4486-7178</orcidid><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 1687-966X
ispartof Stem cells international, 2021-07, Vol.2021, p.6686625-11
issn 1687-966X
1687-9678
1687-9678
language eng
recordid cdi_doaj_primary_oai_doaj_org_article_b85443e15a274f88ab34266920ee2fce
source Wiley Online Library Open Access; Publicly Available Content Database; PubMed Central (PMC)
subjects Albumin
Allergens
Allergic diseases
Allergies
Analysis
Asthma
Body fat
Bronchus
Cell culture
Cytokines
Eosinophils
Extracellular vesicles
Immunoglobulin G
Immunoglobulins
Immunomodulation
Inflammation
Interleukins
Lavage
Leukocytes (eosinophilic)
Lungs
Lymph nodes
Lymphatic system
Lymphocytes
Lymphocytes T
Mice
Ovalbumin
Respiratory tract diseases
Stem cell transplantation
Stem cells
T cells
Vesicles
title Intranasally Administered Extracellular Vesicles from Adipose Stem Cells Have Immunomodulatory Effects in a Mouse Model of Asthma
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-29T18%3A26%3A17IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_doaj_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Intranasally%20Administered%20Extracellular%20Vesicles%20from%20Adipose%20Stem%20Cells%20Have%20Immunomodulatory%20Effects%20in%20a%20Mouse%20Model%20of%20Asthma&rft.jtitle=Stem%20cells%20international&rft.au=Mun,%20Sue%20Jean&rft.date=2021-07-31&rft.volume=2021&rft.spage=6686625&rft.epage=11&rft.pages=6686625-11&rft.issn=1687-966X&rft.eissn=1687-9678&rft_id=info:doi/10.1155/2021/6686625&rft_dat=%3Cgale_doaj_%3EA696974836%3C/gale_doaj_%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c612t-fd0e1f0d90b0a40c212aa0ffe684c97cf1fad7ea4558cbb161e0bd21dcce55bb3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=2609153323&rft_id=info:pmid/34899920&rft_galeid=A696974836&rfr_iscdi=true