Mycolicibacterium fortuitum genomic epidemiology, resistome and virulome

• Published in: Memórias do Instituto Oswaldo Cruz 2021-01, Vol.116, p.e210247-e210247
• Main Authors: Morgado, Sergio, Ramos, Nilcéia de Veiga, Freitas, Fernanda, da Fonseca, Érica Lourenço, Vicente, Ana Carolina
• Format: Article
• Language: English
• Subjects: Antitubercular Agents; arr; Brazil - epidemiology; ESX; Genomics; Humans; opportunist pathogen; PARASITOLOGY; resistance; rifampin; TROPICAL MEDICINE; type VII secretion system; Virulence - genetics
• ISSN: 0074-0276; 1678-8060; 1678-8060
• DOI: 10.1590/0074-02760210247

Mycolicibacterium fortuitum is an opportunistic pathogen associated with human and animal infection worldwide. Studies concerning this species are mainly represented by case reports, some of them addressing drug susceptibility with a focus on a specific geographic region, so there is a gap in relation to the global epidemiological scenario. We aimed determine the global epidemiological scenario of M. fortuitum and analyse its traits associated with pathogenicity. Based on publicly available genomes of M. fortuitum and a genome from Brazil (this study), we performed a genomic epidemiology analysis and in silico and in vitro characterisation of the resistome and virulome of this species. Three main clusters were defined, one including isolates from the environment, human and animal infections recovered over nearly a century. An apparent intrinsic resistome comprises mechanisms associated with macrolides, beta-lactams, aminoglycosides and antitubercular drugs such as rifampin. Besides, the virulome presented Type VII secretion systems (T7SS), including ESX-1, ESX-3, ESX-4 and ESX-4-bis, some of which play a role on the virulence of Mycobacteriaceae species. Here, M. fortuitum was revealed as a reservoir of an expressive intrinsic resistome, as well as a virulome that may contribute to its success as a global opportunist pathogen.