Wound healing potential of human umbilical cord mesenchymal stem cell conditioned medium: An in vitro and in vivo study in diabetes-induced rats

• Published in: Veterinary World 2021-08, Vol.14 (8), p.2109-2117
• Main Authors: Hendrawan, Siufui, Kusnadi, Yuyus, Lagonda, Christine Ayu, Fauza, Dilafitria, Lheman, Jennifer, Budi, Erwin, Manurung, Brian Saputra, Baer, Hans Ulrich, Tansil Tan, Sukmawati
• Format: Article
• Language: English
• Subjects: conditioned medium; diabetic induced rat; human umbilical cord mesenchymal stem cells; wound healing
• ISSN: 0972-8988; 2231-0916
• DOI: 10.14202/vetworld.2021.2109-2117

Human umbilical cord mesenchymal stem cells (hUC-MSCs) and its conditioned medium (CM) promote wound healing. This study investigated the wound healing potential of hUC-MSC CM and using diabetic animal models. The CM from hUC-MSC CM prepared under hypoxic conditions (hypoxic hUC-MSC) was evaluated for stimulating rat fibroblast growth, collagen production ( ), and wound healing in animal models ( ). An excision wound on the dorsal side of the diabetes-induced rats was established, and the rats were randomly divided into non-treatment, antibiotic, and hypoxic hUC-MSC CM groups. The cell number of fibroblasts and collagen secretion was evaluated and compared among the groups in an study. By contrast, wound size reduction, width of re-epithelialization, and the collagen formation area were assessed and compared among the groups in an study. CM under hypoxic conditions contained a higher concentration of wound healing-related growth factors. Hypoxic hUC-MSC CM could facilitate fibroblast cell growth and collagen synthesis, although not significant compared with the control group. Re-epithelialization and collagen production were higher in the hUC-MSC CM group than in the antibiotic and non-treatment groups. Hypoxic hUC-MSC CM possessed more positive effects on the wound healing process based on re-epithelialization and collagen formation than antibiotic treatment did.