Glucosylceramides from Cladosporium and Their Roles in Fungi–Plant Interaction

Cladosporium species are widely distributed filamentous fungi. One of the most important species is C. herbarum, which is related to infections in a variety of plants and of concern in plantations. Fungal cerebrosides, such as glucosylceramide (GlcCer), have been described as playing important roles...

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Published in:Microbiology research 2022-09, Vol.13 (3), p.350-365
Main Authors: Xisto, Mariana Ingrid Dutra da Silva, Bernardino, Mariana Collodetti, Rollin-Pinheiro, Rodrigo, Montebianco, Caroline Barros, da Silva, Andrêina Paula, Calixto, Renata Oliveira Rocha, Mattos, Bianca Braz, Vaslin, Maite Freitas Silva, Barreto-Bergter, Eliana
Format: Article
Language:English
Subjects:
Antibodies
Cellulose acetate
Cladosporium
Cladosporium herbarum
Defense mechanisms
Fatty acids
Fungi
Gene expression
glucosylceramides
Lipids
Liquid oxygen
Mass spectrometry
Mass spectroscopy
Molecular ions
Monoclonal antibodies
Passiflora edulis
Pathogenesis
Pathogens
plant defense-related genes
Plant resistance
Potassium
Sugar
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Summary:Cladosporium species are widely distributed filamentous fungi. One of the most important species is C. herbarum, which is related to infections in a variety of plants and of concern in plantations. Fungal cerebrosides, such as glucosylceramide (GlcCer), have been described as playing important roles in fungal growth and pathogenesis, but GlcCer from C. herbarum has not been characterized so far. For this reason, the present study aimed to elucidate the chemical structure of GlcCer from C. herbarum and its role in the interaction with Passiflora edulis. Mass spectrometry characterization of purified GlcCer revealed two major molecular ions, m/z 760 and m/z 774, and it reacts with monoclonal anti-GlcCer antibodies and is exposed on the fungal surface. P. edulis treatment with GlcCer induced increased levels of superoxide as well as the expression of some genes related to plant defense, such as PR3, POD, LOX and PAL. GlcCer also enhanced growth parameters, such as plant height and root weight. All these results suggest that C. herbarum GlcCer can stimulate plant defense mechanisms, which could help plants to face fungal infections.
ISSN:2036-7481
2036-7481
DOI:10.3390/microbiolres13030028