The Pro12Ala polymorphism in the PPAR-γ2 gene is not associated with an increased risk of NAFLD in Iranian patients with type 2 diabetes mellitus

• Published in: Cellular & molecular biology letters 2019-03, Vol.24 (1), p.12-12, Article 12
• Main Authors: Saremi, Leila, Lotfipanah, Shirin, Mohammadi, Masumeh, Hosseinzadeh, Hassan, Fathi-Kazerooni, Mina, Johari, Behrooz, Saltanatpour, Zohreh
• Format: Article
• Language: English
• Subjects: Biopsy; Body mass index; Cholesterol; Diabetes; Diabetes mellitus (non-insulin dependent); Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - genetics; Female; Gene polymorphism; Genes; Genetic Predisposition to Disease; Genotype & phenotype; Genotyping; Hepatitis; High density lipoprotein; Humans; Insulin resistance; Iran; Lipids; Liver diseases; Male; Metabolic disorders; Metabolic syndrome; Middle Aged; NAFLD; Non-alcoholic Fatty Liver Disease - complications; Non-alcoholic Fatty Liver Disease - genetics; Obesity; Pathogenesis; Patients; Peroxisome proliferator-activated receptors; Polymorphism; Polymorphism, Single Nucleotide - genetics; PPAR gamma - genetics; PPAR­γ2 gene; Pro12Ala polymorphism; Restriction fragment length polymorphism; Risk Factors; Short Report; Statistical analysis; Transcription factors; Type 2 diabetes mellitus; Variables
• ISSN: 1425-8153; 1689-1392
• DOI: 10.1186/s11658-019-0138-0

The peroxisome proliferator-activated receptors (PPARs) are ligand-activated transcription factors that belong to the nuclear hormone receptor superfamily. Several studies have demonstrated a significant association between Pro12Ala polymorphism of the PPAR-γ2 gene and metabolic disorders. Therefore, this study aimed to evaluate the association of Pro12Ala polymorphism with increased risk of NAFLD in Iranian patients with type 2 diabetes mellitus. This cross-sectional study was performed on 145 healthy control subjects and 145 NAFLD patients with a history of type 2 diabetes. Pro12Ala polymorphism genotyping was performed using PCR-restriction fragment length polymorphism (RFLP) technique with the restriction enzyme. Our results demonstrated that CC and GG genotypes of Pro12Ala were found in the participants, but there was no statistically significant difference between NAFLD patients and healthy controls (  = 0.64 and χ  = 0.21). This study suggests that Pro12Ala polymorphism of the PPAR-γ2 gene cannot be considered as a risk factor for NAFLD in the Iranian population.