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Deep sequencing reveals new roles for MuB in transposition immunity and target-capture, and redefines the insular Ter region of E. coli

The target capture protein MuB is responsible for the high efficiency of phage Mu transposition within the genome. However, some targets are off-limits, such as regions immediately outside the Mu ends ( -immunity) as well as the entire ~ 37 kb genome of Mu (Mu genome immunity). Paradoxically, MuB is...

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Published in:Mobile DNA 2020-07, Vol.11 (1), p.26-26, Article 26
Main Authors: Walker, David M, Harshey, Rasika M
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description The target capture protein MuB is responsible for the high efficiency of phage Mu transposition within the genome. However, some targets are off-limits, such as regions immediately outside the Mu ends ( -immunity) as well as the entire ~ 37 kb genome of Mu (Mu genome immunity). Paradoxically, MuB is responsible for -immunity and is also implicated in Mu genome immunity, but via different mechanisms. This study was undertaken to dissect the role of MuB in target choice in vivo. We tracked Mu transposition from six different starting locations on the genome, in the presence and absence of MuB. The data reveal that Mu's ability to sample the entire genome during a single hop in a clonal population is independent of MuB, and that MuB is responsible for -immunity, plays a minor role in Mu genome immunity, and facilitates insertions into transcriptionally active regions. Unexpectedly, transposition patterns in the absence of MuB have helped extend the boundaries of the insular Ter segment of the genome. The results in this study demonstrate unambiguously the operation of two distinct mechanisms of Mu target immunity, only one of which is wholly dependent on MuB. The study also reveals several interesting and hitherto unknown aspects of Mu target choice in vivo, particularly the role of MuB in facilitating the capture of promoter and translation start site targets, likely by displacing macromolecular complexes engaged in gene expression. So also, MuB facilitates transposition into the restricted Ter region of the genome.
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subjects Chromosomes
Deoxyribonucleic acid
DNA
E coli
Escherichia coli
Experiments
Gene loci
Genes
Genomes
Genomics
Plasmids
Transcription (Genetics)
title Deep sequencing reveals new roles for MuB in transposition immunity and target-capture, and redefines the insular Ter region of E. coli
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