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Deep sequencing reveals new roles for MuB in transposition immunity and target-capture, and redefines the insular Ter region of E. coli
The target capture protein MuB is responsible for the high efficiency of phage Mu transposition within the genome. However, some targets are off-limits, such as regions immediately outside the Mu ends ( -immunity) as well as the entire ~ 37 kb genome of Mu (Mu genome immunity). Paradoxically, MuB is...
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Published in: | Mobile DNA 2020-07, Vol.11 (1), p.26-26, Article 26 |
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description | The target capture protein MuB is responsible for the high efficiency of phage Mu transposition within the
genome. However, some targets are off-limits, such as regions immediately outside the Mu ends (
-immunity) as well as the entire ~ 37 kb genome of Mu (Mu genome immunity). Paradoxically, MuB is responsible for
-immunity and is also implicated in Mu genome immunity, but via different mechanisms. This study was undertaken to dissect the role of MuB in target choice in vivo.
We tracked Mu transposition from six different starting locations on the
genome, in the presence and absence of MuB. The data reveal that Mu's ability to sample the entire genome during a single hop in a clonal population is independent of MuB, and that MuB is responsible for
-immunity, plays a minor role in Mu genome immunity, and facilitates insertions into transcriptionally active regions. Unexpectedly, transposition patterns in the absence of MuB have helped extend the boundaries of the insular Ter segment of the
genome.
The results in this study demonstrate unambiguously the operation of two distinct mechanisms of Mu target immunity, only one of which is wholly dependent on MuB. The study also reveals several interesting and hitherto unknown aspects of Mu target choice in vivo, particularly the role of MuB in facilitating the capture of promoter and translation start site targets, likely by displacing macromolecular complexes engaged in gene expression. So also, MuB facilitates transposition into the restricted Ter region of the genome. |
doi_str_mv | 10.1186/s13100-020-00217-9 |
format | article |
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genome. However, some targets are off-limits, such as regions immediately outside the Mu ends (
-immunity) as well as the entire ~ 37 kb genome of Mu (Mu genome immunity). Paradoxically, MuB is responsible for
-immunity and is also implicated in Mu genome immunity, but via different mechanisms. This study was undertaken to dissect the role of MuB in target choice in vivo.
We tracked Mu transposition from six different starting locations on the
genome, in the presence and absence of MuB. The data reveal that Mu's ability to sample the entire genome during a single hop in a clonal population is independent of MuB, and that MuB is responsible for
-immunity, plays a minor role in Mu genome immunity, and facilitates insertions into transcriptionally active regions. Unexpectedly, transposition patterns in the absence of MuB have helped extend the boundaries of the insular Ter segment of the
genome.
The results in this study demonstrate unambiguously the operation of two distinct mechanisms of Mu target immunity, only one of which is wholly dependent on MuB. The study also reveals several interesting and hitherto unknown aspects of Mu target choice in vivo, particularly the role of MuB in facilitating the capture of promoter and translation start site targets, likely by displacing macromolecular complexes engaged in gene expression. So also, MuB facilitates transposition into the restricted Ter region of the genome.</description><identifier>ISSN: 1759-8753</identifier><identifier>EISSN: 1759-8753</identifier><identifier>DOI: 10.1186/s13100-020-00217-9</identifier><identifier>PMID: 32670425</identifier><language>eng</language><publisher>England: BioMed Central Ltd</publisher><subject>Chromosomes ; Deoxyribonucleic acid ; DNA ; E coli ; Escherichia coli ; Experiments ; Gene loci ; Genes ; Genomes ; Genomics ; Plasmids ; Transcription (Genetics)</subject><ispartof>Mobile DNA, 2020-07, Vol.11 (1), p.26-26, Article 26</ispartof><rights>The Author(s) 2020.</rights><rights>COPYRIGHT 2020 BioMed Central Ltd.</rights><rights>2020. This work is licensed under http://creativecommons.org/licenses/by/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>The Author(s) 2020</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c563t-951907f8f3b4d7dabac22d1a09db995de92e8ec01468521239ec5419967ef0b3</citedby><cites>FETCH-LOGICAL-c563t-951907f8f3b4d7dabac22d1a09db995de92e8ec01468521239ec5419967ef0b3</cites><orcidid>0000-0002-2785-0879</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC7350765/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.proquest.com/docview/2424817224?pq-origsite=primo$$EHTML$$P50$$Gproquest$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,881,25728,27898,27899,36986,36987,44563,53763,53765</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/32670425$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Walker, David M</creatorcontrib><creatorcontrib>Harshey, Rasika M</creatorcontrib><title>Deep sequencing reveals new roles for MuB in transposition immunity and target-capture, and redefines the insular Ter region of E. coli</title><title>Mobile DNA</title><addtitle>Mob DNA</addtitle><description>The target capture protein MuB is responsible for the high efficiency of phage Mu transposition within the
genome. However, some targets are off-limits, such as regions immediately outside the Mu ends (
-immunity) as well as the entire ~ 37 kb genome of Mu (Mu genome immunity). Paradoxically, MuB is responsible for
-immunity and is also implicated in Mu genome immunity, but via different mechanisms. This study was undertaken to dissect the role of MuB in target choice in vivo.
We tracked Mu transposition from six different starting locations on the
genome, in the presence and absence of MuB. The data reveal that Mu's ability to sample the entire genome during a single hop in a clonal population is independent of MuB, and that MuB is responsible for
-immunity, plays a minor role in Mu genome immunity, and facilitates insertions into transcriptionally active regions. Unexpectedly, transposition patterns in the absence of MuB have helped extend the boundaries of the insular Ter segment of the
genome.
The results in this study demonstrate unambiguously the operation of two distinct mechanisms of Mu target immunity, only one of which is wholly dependent on MuB. The study also reveals several interesting and hitherto unknown aspects of Mu target choice in vivo, particularly the role of MuB in facilitating the capture of promoter and translation start site targets, likely by displacing macromolecular complexes engaged in gene expression. So also, MuB facilitates transposition into the restricted Ter region of the genome.</description><subject>Chromosomes</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>E coli</subject><subject>Escherichia coli</subject><subject>Experiments</subject><subject>Gene loci</subject><subject>Genes</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Plasmids</subject><subject>Transcription (Genetics)</subject><issn>1759-8753</issn><issn>1759-8753</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2020</creationdate><recordtype>article</recordtype><sourceid>PIMPY</sourceid><sourceid>DOA</sourceid><recordid>eNptks1u1DAUhSMEolXpC7BAltiwIIP_HW-Q2lKgUhGb2Vse5yb1KLEHOynqE_S165kppYNwZCW6Pve78smpqrcELwhp5KdMGMG4xrRsTImq9YvqmCih60YJ9vLZ91F1mvMab5fCpOGvqyNGpcKciuPq_gvABmX4NUNwPvQowS3YIaMAv1GKA2TUxYR-zOfIBzQlG_ImZj_5GJAfxzn46Q7Z0KLJph6m2tnNNCf4uKslaKHzoTCmGyj9eR5sQktI5aTfEmKHLhfIxcG_qV51ZSycPr5PquXXy-XF9_r657eri7Pr2gnJploLorHqmo6teKtau7KO0pZYrNuV1qIFTaEBhwmXjaCEMg1OcKK1VNDhFTuprvbYNtq12SQ_2nRnovVmV4ipNzZN3g1gHLZaaEWlY5J3tm04aTDT0nLRUS51YX3eszbzaoTWQSj2DAfQw5Pgb0wfb41iAispCuDDIyDF4n-ezOizg2GwAeKcDeWUcyaUZEX6_h_pOs4pFKd2qoYoSvlfVW_LBXzoYpnrtlBzJhlpNCGCFtXiP6rytDB6F0P5ZaV-0ED3DS7FnBN0T3ck2GzDaPZhNCWMZhdGs3Xn3XN3nlr-RI89AMYD2OU</recordid><startdate>20200709</startdate><enddate>20200709</enddate><creator>Walker, David M</creator><creator>Harshey, Rasika M</creator><general>BioMed Central Ltd</general><general>BioMed Central</general><general>BMC</general><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7X7</scope><scope>7XB</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>K9.</scope><scope>M0S</scope><scope>PHGZM</scope><scope>PHGZT</scope><scope>PIMPY</scope><scope>PKEHL</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope><orcidid>https://orcid.org/0000-0002-2785-0879</orcidid></search><sort><creationdate>20200709</creationdate><title>Deep sequencing reveals new roles for MuB in transposition immunity and target-capture, and redefines the insular Ter region of E. coli</title><author>Walker, David M ; Harshey, Rasika M</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c563t-951907f8f3b4d7dabac22d1a09db995de92e8ec01468521239ec5419967ef0b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2020</creationdate><topic>Chromosomes</topic><topic>Deoxyribonucleic acid</topic><topic>DNA</topic><topic>E coli</topic><topic>Escherichia coli</topic><topic>Experiments</topic><topic>Gene loci</topic><topic>Genes</topic><topic>Genomes</topic><topic>Genomics</topic><topic>Plasmids</topic><topic>Transcription (Genetics)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Walker, David M</creatorcontrib><creatorcontrib>Harshey, Rasika M</creatorcontrib><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Health Medical collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>ProQuest Central (New)</collection><collection>ProQuest One Academic (New)</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Middle East (New)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>Directory of Open Access Journals</collection><jtitle>Mobile DNA</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Walker, David M</au><au>Harshey, Rasika M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Deep sequencing reveals new roles for MuB in transposition immunity and target-capture, and redefines the insular Ter region of E. coli</atitle><jtitle>Mobile DNA</jtitle><addtitle>Mob DNA</addtitle><date>2020-07-09</date><risdate>2020</risdate><volume>11</volume><issue>1</issue><spage>26</spage><epage>26</epage><pages>26-26</pages><artnum>26</artnum><issn>1759-8753</issn><eissn>1759-8753</eissn><abstract>The target capture protein MuB is responsible for the high efficiency of phage Mu transposition within the
genome. However, some targets are off-limits, such as regions immediately outside the Mu ends (
-immunity) as well as the entire ~ 37 kb genome of Mu (Mu genome immunity). Paradoxically, MuB is responsible for
-immunity and is also implicated in Mu genome immunity, but via different mechanisms. This study was undertaken to dissect the role of MuB in target choice in vivo.
We tracked Mu transposition from six different starting locations on the
genome, in the presence and absence of MuB. The data reveal that Mu's ability to sample the entire genome during a single hop in a clonal population is independent of MuB, and that MuB is responsible for
-immunity, plays a minor role in Mu genome immunity, and facilitates insertions into transcriptionally active regions. Unexpectedly, transposition patterns in the absence of MuB have helped extend the boundaries of the insular Ter segment of the
genome.
The results in this study demonstrate unambiguously the operation of two distinct mechanisms of Mu target immunity, only one of which is wholly dependent on MuB. The study also reveals several interesting and hitherto unknown aspects of Mu target choice in vivo, particularly the role of MuB in facilitating the capture of promoter and translation start site targets, likely by displacing macromolecular complexes engaged in gene expression. So also, MuB facilitates transposition into the restricted Ter region of the genome.</abstract><cop>England</cop><pub>BioMed Central Ltd</pub><pmid>32670425</pmid><doi>10.1186/s13100-020-00217-9</doi><tpages>1</tpages><orcidid>https://orcid.org/0000-0002-2785-0879</orcidid><oa>free_for_read</oa></addata></record> |
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subjects | Chromosomes Deoxyribonucleic acid DNA E coli Escherichia coli Experiments Gene loci Genes Genomes Genomics Plasmids Transcription (Genetics) |
title | Deep sequencing reveals new roles for MuB in transposition immunity and target-capture, and redefines the insular Ter region of E. coli |
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