Organ-specific, multimodal, wireless optoelectronics for high-throughput phenotyping of peripheral neural pathways

• Published in: Nature communications 2021-01, Vol.12 (1), p.157-157, Article 157
• Main Authors: Kim, Woo Seok, Hong, Sungcheol, Gamero, Milenka, Jeevakumar, Vivekanand, Smithhart, Clay M., Price, Theodore J., Palmiter, Richard D., Campos, Carlos, Park, Sung Il
• Format: Article
• Language: English
• Subjects: 38; 631/1647/2253; 631/378/1488/393; 631/378/1959/2605; 631/378/3917; 631/61/54/993; 82/80; Animals; Appetite; Appetite - physiology; Autonomic nervous system; Behavior Observation Techniques - instrumentation; Calcitonin Gene-Related Peptide - genetics; Chemoreceptor Cells - physiology; Coils; Durability; Equipment Design; Female; Fibers; High-Throughput Screening Assays - instrumentation; Humanities and Social Sciences; Light sources; Male; Mice, Transgenic; Models, Animal; multidisciplinary; Multiplexing; Neural networks; Neural Pathways - physiology; Optoelectronic devices; Optogenetics - instrumentation; Organs; Phenotyping; Physiological effects; Physiology; Remote Sensing Technology - instrumentation; Science; Science (multidisciplinary); Sensory neurons; Stomach - cytology; Stomach - innervation; Stomach - physiology; Telemetry; Vagus nerve; Vagus Nerve - cytology; Vagus Nerve - physiology; Wireless Technology - instrumentation
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-020-20421-8

The vagus nerve supports diverse autonomic functions and behaviors important for health and survival. To understand how specific components of the vagus contribute to behaviors and long-term physiological effects, it is critical to modulate their activity with anatomical specificity in awake, freely behaving conditions using reliable methods. Here, we introduce an organ-specific scalable, multimodal, wireless optoelectronic device for precise and chronic optogenetic manipulations in vivo. When combined with an advanced, coil-antenna system and a multiplexing strategy for powering 8 individual homecages using a single RF transmitter, the proposed wireless telemetry enables low cost, high-throughput, and precise functional mapping of peripheral neural circuits, including long-term behavioral and physiological measurements. Deployment of these technologies reveals an unexpected role for stomach, non-stretch vagal sensory fibers in suppressing appetite and demonstrates the durability of the miniature wireless device inside harsh gastric conditions. Advances in wireless technologies have enabled internalisation of light sources, but organ specific illumination is challenging. Here, the authors present a durable, multimodal, wireless system enabling optogenetic stimulation of peripheral neurons within organs.