Mastrevirus Rep and RepA Proteins Suppress de novo Transcriptional Gene Silencing

Transcriptional gene silencing (TGS) in plants is a defense mechanism against DNA virus infection. The genomes of viruses in the family encode several TGS suppressors. In this study, we induced de novo TGS against the transgenic gene encoding green fluorescent protein by expressing a hairpin-shaped...

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Bibliographic Details
Published in:International journal of molecular sciences 2021-10, Vol.22 (21), p.11462
Main Authors: Watanabe, Kikyo, Ugaki, Masashi
Format: Article
Language:English
Subjects:
DNA methylation
DNA viruses
Fluorescence
Geminiviridae - metabolism
Geminivirus
Gene Silencing
Genes
Genomes
Green fluorescent protein
Host Microbial Interactions
Plant Diseases - virology
Plant virus diseases
Plants - virology
Proteins
Rep
RepA
RNA polymerase
silencing suppressor
siRNA
transcriptional gene silencing
TYDV
Viral Proteins - metabolism
Viruses
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Summary:Transcriptional gene silencing (TGS) in plants is a defense mechanism against DNA virus infection. The genomes of viruses in the family encode several TGS suppressors. In this study, we induced de novo TGS against the transgenic gene encoding green fluorescent protein by expressing a hairpin-shaped self-complementary RNA corresponding to the enhancer region of the 35S promoter (hpE35S). In addition, we examined the TGS suppression activity of proteins encoded in the genome of (TYDV, genus ). The results show that the replication-associated protein (Rep) and RepA encoded by TYDV have TGS suppressor activity and lead to decreased accumulation of 24-nt siRNAs. These results suggest that Rep and RepA can block the steps before the loading of siRNAs into Argonaute (AGO) proteins. This is the first report of TGS suppressors in the genus .
ISSN:1422-0067
1661-6596
1422-0067
DOI:10.3390/ijms222111462