Supercritical Fluid Extract of Angelica sinensis and Zingiber officinale Roscoe Ameliorates TNBS-Induced Colitis in Rats

• Published in: International journal of molecular sciences 2019-08, Vol.20 (15), p.3816
• Main Authors: Liu, Jia, Yu, Ling, Mo, Nuolan, Lan, Hai, Zhang, Yan, Liu, Xin, Wu, Qing
• Format: Article
• Language: English
• Subjects: Angelica sinensis; Angelica sinensis - chemistry; Animals; anti-inflammatory; Anti-Inflammatory Agents, Non-Steroidal - chemistry; Anti-Inflammatory Agents, Non-Steroidal - isolation & purification; Anti-Inflammatory Agents, Non-Steroidal - pharmacology; Biomarkers; Biopsy; Cancer therapies; Cell Survival - drug effects; Chromatography, Supercritical Fluid; Colitis; Colitis - drug therapy; Colitis - etiology; Colitis - metabolism; Colorectal cancer; Cytokines - biosynthesis; Design of experiments; Disease Models, Animal; Drug dosages; Drugs; Experiments; Gas Chromatography-Mass Spectrometry; Gingerol; Inflammation; Inflammation Mediators - metabolism; Inflammatory bowel disease; Lipopolysaccharides; Lipopolysaccharides - immunology; Lymphocytes - immunology; Lymphocytes - metabolism; Mice; Nitric Oxide - metabolism; Optimization; Organic chemistry; Oxidative stress; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Process parameters; Rats; RAW 264.7 Cells; Spleen - immunology; Spleen - metabolism; Studies; supercritical fluid extract; Supercritical fluids; Trinitrobenzenesulfonic Acid - adverse effects; Zingiber officinale; Zingiber officinale - chemistry; Zingiber officinale Roscoe
• ISSN: 1422-0067; 1661-6596; 1422-0067
• DOI: 10.3390/ijms20153816

Inflammatory bowel disease (IBD) is a worldwide healthcare problem calling for the development of new therapeutic drugs. and Roscoe are two common dietetic Chinese herbs, which are traditionally used for complementary treatment of gastrointestinal disorders. As bioactive constituents, volatile and pungent substances of these two herbs could be effectively extracted together by supercritical fluid extraction. In this study, the supercritical fluid extract of and Roscoe (AZ-SFE) was obtained by an optimized extraction process and it was chemically characterized. The anti-inflammatory effect and underlying mechanism of AZ-SFE were evaluated in a lipopolysaccharide (LPS)-induced RAW264.7 cell model and a 2, 4, 6-trinitrobenzenesulfonic acid (TNBS)-induced colitis rat model. AZ-SFE notably inhibited the production of NO in LPS-stimulated macrophages, and it inhibited the proliferation of Concanavalin A (Con A)-induced splenocytes with suppression of the Th1 immune response. In vivo, the study demonstrated that AZ-SFE significantly alleviated disease activity, colonic shortening, macroscopic damage and histological injury of TNBS-treated rats with reduction of oxidative stress, suppression of inflammatory cytokines, and modulation of hepcidin and serum iron. These findings suggested that AZ-SFE may be a promising supplement for current IBD therapy.