Transcriptional Expression of the ompA , cpaf , tarp, and tox Genes of Chlamydia trachomatis Clinical Isolates at Different Stages of the Developmental Cycle

The transcriptional gene expression patterns of have mainly been studied using reference strains propagated in cultured cells. Here, using five low-passage-number clinical isolates that originated from asymptomatic or symptomatic female patients, the in vitro expression of the , , , and genes was st...

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Bibliographic Details
Published in:Microorganisms (Basel) 2019-05, Vol.7 (6), p.153
Main Authors: Korhonen, Suvi, Hokynar, Kati, Mannonen, Laura, Paavonen, Jorma, Hiltunen-Back, Eija, Puolakkainen, Mirja
Format: Article
Language:English
Subjects:
Bacterial infections
Cell cycle
Chlamydia
Chlamydia trachomatis
Clinical isolates
Gene expression
Genes
Genomes
Growth kinetics
Infections
Kinases
Laboratories
low-passage-number isolates
Performance evaluation
RNA-directed DNA polymerase
Sexually transmitted diseases
STD
Thermal cycling
Transcription
transcriptional expression
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Summary:The transcriptional gene expression patterns of have mainly been studied using reference strains propagated in cultured cells. Here, using five low-passage-number clinical isolates that originated from asymptomatic or symptomatic female patients, the in vitro expression of the , , , and genes was studied with reverse transcriptase real-time PCR during the chlamydial developmental cycle. We observed dissimilarities in the gene expression patterns between the low-passage-number clinical isolates and the reference strains. The expression of and the peak of the expression were observed earlier in the reference strains than in most of the clinical isolates. The expression of was high in the reference strains compared with the clinical isolates at the mid-phase (6-24 hours post infection) of the developmental cycle. All of the strains had a rather similar expression profile. Four out of five clinical isolates exhibited slower growth kinetics compared with the reference strains. The use of low-passage-number clinical isolates instead of reference strains in the studies might better reflect the situation in human infection.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms7060153