Microbial interaction-induced siderophore dynamics lead to phenotypic differentiation of Staphylococcus aureus

This study investigated the impact of microbial interactions on siderophore dynamics and phenotypic differentiation of under iron-deficient conditions. Optimization of media demonstrated that the glycerol alanine salts medium was best suited for analyzing the dynamics of siderophore production becau...

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Bibliographic Details
Published in:Frontiers in cellular and infection microbiology 2023, Vol.13, p.1277176-1277176
Main Authors: Rajapitamahuni, Soundarya, Lyou, Eun Sun, Kang, Bo Ram, Lee, Tae Kwon
Format: Article
Language:English
Subjects:
Escherichia coli
Humans
Iron
iron deficiency
microbial interactions
phenotype
Raman spectroscopy
Siderophores
Staphylococcal Infections
Staphylococcus aureus
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Summary:This study investigated the impact of microbial interactions on siderophore dynamics and phenotypic differentiation of under iron-deficient conditions. Optimization of media demonstrated that the glycerol alanine salts medium was best suited for analyzing the dynamics of siderophore production because of its stable production of diverse siderophore types. The effects of pH and iron concentration on siderophore yield revealed a maximum yield at neutral pH and low iron concentration (10 µg). Microbial interaction studies have highlighted variations in siderophore production when different strains ( , , and ) are co-cultured with . Co-culture of with eliminated siderophore production in , while co-culture of with and produced one or two siderophores, respectively. Raman spectroscopy revealed that microbial interactions and siderophore dynamics play a crucial role in directing the phenotypic differentiation of , especially under iron-deficient conditions. Our results suggest that microbial interactions profoundly influence siderophore dynamics and phenotypic differentiation and that the study of these interactions could provide valuable insights for understanding microbial survival strategies in iron-limited environments.
ISSN:2235-2988
2235-2988
DOI:10.3389/fcimb.2023.1277176