Simultaneous species detection and discovery with environmental DNA metabarcoding: A freshwater mollusk case study

Environmental DNA (eDNA) sampling is a powerful tool for rapidly characterizing biodiversity patterns for specious, cryptic taxa with incomplete taxonomies. One such group that are also of high conservation concern are North American freshwater gastropods. In particular, springsnails of the genus Py...

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Bibliographic Details
Published in:Ecology and evolution 2024-02, Vol.14 (2), p.e11020-n/a
Main Authors: Vanderpool, Daniel D., Wilcox, Taylor M., Young, Michael K., Pilgrim, Kristine L., Schwartz, Michael K.
Format: Article
Language:English
Subjects:
Biodiversity
Case studies
Climate change
Conservation
DNA barcoding
Endangered & extinct species
Endangered species
Environmental DNA
environmental DNA species detection
Freshwater mollusks
Gastropoda
Genetic diversity
Genetic engineering
Geographical distribution
Groundwater
Groundwater mining
Habitat loss
Haplotypes
metabarcoding
Mollusks
Morphology
Phylogenetics
Pyrgulopsis
Sampling
Sampling methods
Snails
springsnails
Taxa
Taxonomy
Water purification
Wildlife conservation
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Summary:Environmental DNA (eDNA) sampling is a powerful tool for rapidly characterizing biodiversity patterns for specious, cryptic taxa with incomplete taxonomies. One such group that are also of high conservation concern are North American freshwater gastropods. In particular, springsnails of the genus Pyrgulopsis (Family: Hydrobiidae) are prevalent throughout the western United States where >140 species have been described. Many of the described species are narrow endemics known from a single spring or locality, and it is believed that there are likely many additional species which have yet to be described. The distribution of these species across the landscape is of interest because habitat loss and degradation, climate change, groundwater mining, and pollution have resulted in springsnail imperilment rates as high as 92%. Determining distributions with conventional sampling methods is limited by the fact that these snails are often 140 species have been described. Many of the described species are narrow endemics known from a single spring or locality, and it is believed that there are likely many additional species which have yet to be described. We developed a metabarcoding strategy that uses modified COI primers for sensitive detection of Pyrgulopsis sp. springsnails that can be rapidly deployed at landscape scales for use
ISSN:2045-7758
2045-7758
DOI:10.1002/ece3.11020