Optogenetic manipulation of neuronal and cardiomyocyte functions in zebrafish using microbial rhodopsins and adenylyl cyclases

Even though microbial photosensitive proteins have been used for optogenetics, their use should be optimized to precisely control cell and tissue functions in vivo. We exploited Gt CCR4 and Kn ChR, cation channelrhodopsins from algae, Be GC1, a guanylyl cyclase rhodopsin from a fungus, and photoacti...

Full description

Saved in:
Bibliographic Details
Published in:eLife 2023-08, Vol.12
Main Authors: Hagio, Hanako, Koyama, Wataru, Hosaka, Shiori, Song, Aysenur Deniz, Narantsatsral, Janchiv, Matsuda, Koji, Shimizu, Takashi, Hososhima, Shoko, Tsunoda, Satoshi P, Kandori, Hideki, Hibi, Masahiko
Format: Article
Language:English
Subjects:
adenylyl cyclase
Algae
Bradycardia
Calcium (intracellular)
cardiac contraction
Cardiomyocytes
Cell Biology
Danio rerio
Depolarization
Genetics
Guanylate cyclase
guanylyl cyclase
Heart
Hindbrain
Information processing
Ions
Locomotion
Neuroscience
Optics
optogenetics
Proteins
Rhodopsin
Swimming behavior
Tools and Resources
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Even though microbial photosensitive proteins have been used for optogenetics, their use should be optimized to precisely control cell and tissue functions in vivo. We exploited Gt CCR4 and Kn ChR, cation channelrhodopsins from algae, Be GC1, a guanylyl cyclase rhodopsin from a fungus, and photoactivated adenylyl cyclases (PACs) from cyanobacteria ( Oa PAC) or bacteria ( b PAC), to control cell functions in zebrafish. Optical activation of Gt CCR4 and Kn ChR in the hindbrain reticulospinal V2a neurons, which are involved in locomotion, induced swimming behavior at relatively short latencies, whereas activation of Be GC1 or PACs achieved it at long latencies. Activation of Gt CCR4 and Kn ChR in cardiomyocytes induced cardiac arrest, whereas activation of b PAC gradually induced bradycardia. Kn ChR activation led to an increase in intracellular Ca 2+ in the heart, suggesting that depolarization caused cardiac arrest. These data suggest that these optogenetic tools can be used to reveal the function and regulation of zebrafish neurons and cardiomyocytes.
ISSN:2050-084X
2050-084X
DOI:10.7554/eLife.83975