NCOA3 knockdown delays human embryo development

• Published in: Heliyon 2024-09, Vol.10 (18), p.e37639, Article e37639
• Main Authors: Wu, Zhaoting, Ma, Xueshan, Wang, Jingyu
• Format: Article
• Language: English
• Subjects: carcinogenesis; Embryo development; embryo transfer; embryogenesis; family; fluorescent antibody technique; hospitals; humans; Metabolism; mice; NANOG; NCOA3; oocytes; quantitative polymerase chain reaction; RNA; Single-oocyte RNA sequencing
• ISSN: 2405-8440; 2405-8440
• DOI: 10.1016/j.heliyon.2024.e37639

Embryonic development is a precisely controlled sequential process influenced by complex external and internal factors; therefore, this process holds paramount significance in the context of in vitro fertilization and embryo transfer (IVF-ET), with internal oocyte and embryo quality being pivotal in determining its success. Nuclear receptor coactivator 3 (NCOA3), a member of the p160 nuclear receptor coactivators family, has been extensively studied in tumorigenesis and reportedly plays a crucial role in maintaining pluripotency in mouse embryonic stem cells (ESCs). However, its functions in human embryo development remain largely unexplored. In this study, we collected human samples, including oocytes, zygotes, and embryos, from patients at the First Affiliated Hospital of Zhengzhou University to investigate whether NCOA3 regulates human embryonic development. To this end, we employed various assays, including immunofluorescence, quantitative real-time PCR (qPCR), microinjection, and RNA sequencing. Our findings suggested that NCOA3 expression level was low in inferior embryos (with >50 % fragmentation), and its presence is closely related to the expression of the pluripotency factor NANOG. Deletion of NCOA3 delays human embryonic development. Single-oocyte RNA sequencing revealed that NCOA3 primarily participates in metabolic alterations in oocytes. In sum, these findings elucidate the pivotal roles of NCOA3 in human embryonic development-NCOA3 deletion compromise the developmental potential of embryos. These mechanistic insights into the role of NCOA3 in human embryonic development not only advances our understanding of the intricate molecular mechanisms involved but also holds potential implications for improving assisted reproductive technologies (ART) and addressing developmental disorders in human embryos.