Heterogeneous force response of chromatin in isolated nuclei

A quantitative description of nuclear mechanics is crucial for understanding its role in force sensing within eukaryotic cells. Recent studies indicate that the chromatin within the nucleus cannot be treated as a homogeneous material. To elucidate its material properties, we combine optical tweezers...

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Bibliographic Details
Published in:Cell reports (Cambridge) 2024-10, Vol.43 (10), p.114852, Article 114852
Main Authors: Bergamaschi, Giulia, Biebricher, Andreas S., Witt, Hannes, Byfield, Fitzroy J., Seymonson, Xamanie M.R., Storm, Cornelis, Janmey, Paul A., Wuite, Gijs J.L.
Format: Article
Language:English
Subjects:
Animals
Cell Nucleus - metabolism
Chromatin - metabolism
CP: Cell biology
CP: Molecular biology
force spectroscopy
Humans
Lamins - metabolism
Mechanobiology
Micrococcal Nuclease - metabolism
Nuclear Envelope - metabolism
nuclear mechanics
Optical Tweezers
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Summary:A quantitative description of nuclear mechanics is crucial for understanding its role in force sensing within eukaryotic cells. Recent studies indicate that the chromatin within the nucleus cannot be treated as a homogeneous material. To elucidate its material properties, we combine optical tweezers manipulation of isolated nuclei with multi-color fluorescence imaging of lamin and chromatin to map the response of nuclei to local deformations. Force spectroscopy reveals nuclear strain stiffening and an exponential force dependence, well described by a hierarchical chain model. Simultaneously, fluorescence data show a higher compliance of chromatin compared to the nuclear envelope at strains
ISSN:2211-1247
2211-1247
DOI:10.1016/j.celrep.2024.114852