Expression of Cell-Cycle Regulatory Proteins pRb, Cyclin D1, and p53 Is Not Associated with Recurrence Rates of Equine Sarcoids

Sarcoids are among the most common tumors diagnosed in equids; their association with bovine papillomaviruses (BPV) infection has been widely reported, but the mechanism of carcinogenesis has not been fully elucidated. To verify whether BPV infection causes dysregulation of the pRb-Cyclin D1-p16CDKN...

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Published in:Veterinary sciences 2022-09, Vol.9 (9), p.474
Main Authors: Tura, Giorgia, Brunetti, Barbara, Brigandì, Elena, Rinnovati, Riccardo, Sarli, Giuseppe, Avallone, Giancarlo, Muscatello, Luisa Vera, La Ragione, Roberto Marcello, Durham, Andy E., Bacci, Barbara
Format: Article
Language:English
Subjects:
Antibodies
Antigens
Binding sites
Biopsy
Biotechnology
bovine papillomavirus
Carcinogenesis
Cell cycle
Cloning
Cyclin D1
equid
Human papillomavirus
Immunohistochemistry
Investigations
p16CDKN2A
p53 Protein
pRb
Proteins
Regulatory proteins
sarcoid
Statistical analysis
Tumorigenesis
Tumors
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Summary:Sarcoids are among the most common tumors diagnosed in equids; their association with bovine papillomaviruses (BPV) infection has been widely reported, but the mechanism of carcinogenesis has not been fully elucidated. To verify whether BPV infection causes dysregulation of the pRb-Cyclin D1-p16CDKN2A-p53 pathway as reported for human papillomavirus (HPV), the study employed immunohistochemistry to test 55 equine sarcoid biopsies for the expression of pRb, Cyclin D1, and p53 cell cycle regulatory proteins and to evaluate the proliferative rate through Ki67. High Cyclin D1 and pRb expression were observed in 51% and 80% of cases, respectively, while low expression was observed in 49% and 20% of cases, respectively. Significantly higher Ki67 proliferation indexes were observed in fibroblastic, nodular, and mixed sarcoids compared to the occult and verrucous. High proliferation was significantly associated with high Cyclin D1 expression. In contrast with previous studies, p53 positivity was not observed in the cases examined in this study. Moreover, follow-up analysis revealed that fibroblastic, mixed sarcoids were associated with significantly higher local recurrence rates while the verrucous subtype was associated with higher rates of new sarcoid development at distant sites.
ISSN:2306-7381
2306-7381
DOI:10.3390/vetsci9090474