Identical folds used for distinct mechanical functions of the bacterial flagellar rod and hook

• Published in: Nature communications 2017-01, Vol.8 (1), p.14276-14276, Article 14276
• Main Authors: Fujii, Takashi, Kato, Takayuki, Hiraoka, Koichi D., Miyata, Tomoko, Minamino, Tohru, Chevance, Fabienne F. V., Hughes, Kelly T., Namba, Keiichi
• Format: Article
• Language: English
• Subjects: 101/28; 631/326/41/2536; 631/535/1258/1259; 631/57/2272/2276; Adapter proteins; Bacteria; Bacterial Outer Membrane Proteins - chemistry; Bacterial Outer Membrane Proteins - genetics; Bacterial Outer Membrane Proteins - metabolism; Bacterial Outer Membrane Proteins - ultrastructure; Bacterial Proteins - chemistry; Bacterial Proteins - genetics; Bacterial Proteins - metabolism; Bacterial Proteins - ultrastructure; Cryoelectron Microscopy; Flagella - physiology; Flagella - ultrastructure; Humanities and Social Sciences; Imaging, Three-Dimensional; Mechanical properties; Molecular Docking Simulation; Molecular Motor Proteins - chemistry; Molecular Motor Proteins - genetics; Molecular Motor Proteins - metabolism; Molecular Motor Proteins - ultrastructure; multidisciplinary; Protein Domains - physiology; Protein Structure, Secondary - physiology; Salmonella typhimurium - cytology; Salmonella typhimurium - physiology; Science; Science (multidisciplinary); Sequence Alignment
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/ncomms14276

The bacterial flagellum is a motile organelle driven by a rotary motor, and its axial portions function as a drive shaft (rod), a universal joint (hook) and a helical propeller (filament). The rod and hook are directly connected to each other, with their subunit proteins FlgG and FlgE having 39% sequence identity, but show distinct mechanical properties; the rod is straight and rigid as a drive shaft whereas the hook is flexible in bending as a universal joint. Here we report the structure of the rod and comparison with that of the hook. While these two structures have the same helical symmetry and repeat distance and nearly identical folds of corresponding domains, the domain orientations differ by ∼7°, resulting in tight and loose axial subunit packing in the rod and hook, respectively, conferring the rigidity on the rod and flexibility on the hook. This provides a good example of versatile use of a protein structure in biological organisms. The bacterial flagellum is a motile organelle that enables bacterial movement. Here the authors explain how the structurally similar flagellum components FlgG and FlgE can give rise to distinct macrostructures—the rod and hook—through subtle differences in domain orientation attributable to a short N-terminal insertion in FlgG.