CALHM1-Mediated ATP Release and Ciliary Beat Frequency Modulation in Nasal Epithelial Cells

Mechanical stimulation of airway epithelial cells causes apical release of ATP, which increases ciliary beat frequency (CBF) and speeds up mucociliary clearance. The mechanisms responsible for this ATP release are poorly understood. CALHM1, a transmembrane protein with shared structural features to...

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Bibliographic Details
Published in:Scientific reports 2017-07, Vol.7 (1), p.6687-8, Article 6687
Main Authors: Workman, Alan D., Carey, Ryan M., Chen, Bei, Saunders, Cecil J., Marambaud, Philippe, Mitchell, Claire H., Tordoff, Michael G., Lee, Robert J., Cohen, Noam A.
Format: Article
Language:English
Subjects:
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ATP
Connexins
Epithelial cells
Frequency dependence
Humanities and Social Sciences
Mechanical stimuli
multidisciplinary
Respiratory tract
Rodents
Science
Science (multidisciplinary)
Taste buds
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Summary:Mechanical stimulation of airway epithelial cells causes apical release of ATP, which increases ciliary beat frequency (CBF) and speeds up mucociliary clearance. The mechanisms responsible for this ATP release are poorly understood. CALHM1, a transmembrane protein with shared structural features to connexins and pannexins, has been implicated in ATP release from taste buds, but it has not been evaluated for a functional role in the airway. In the present study, Calhm1 knockout, Panx1 knockout, and wild-type mouse nasal septal epithelial cells were grown at an air-liquid interface (ALI) and subjected to light mechanical stimulation from an air puff. Apical ATP release was attenuated in Calhm1 knockout cultures following mechanical stimulation at a pressure of 55 mmHg for 50 milliseconds (p 
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-017-07221-9