Complete genomic sequencing of canine distemper virus with nanopore technology during an epizootic event

Canine distemper virus (CDV) endangers a wide range of wild animal populations, can cross species barriers and therefore representing a significant conservational and animal health risk around the globe. During spring to autumn 2021, according to our current estimates a minimum of 50 red foxes ( Vul...

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Bibliographic Details
Published in:Scientific reports 2022-03, Vol.12 (1), p.4116-4116, Article 4116
Main Authors: Lanszki, Zsófia, Tóth, Gábor E., Schütz, Éva, Zeghbib, Safia, Rusvai, Miklós, Jakab, Ferenc, Kemenesi, Gábor
Format: Article
Language:English
Subjects:
631/326/2521
631/326/596
Animal populations
Animals
Canine distemper
Distemper - diagnosis
Distemper - epidemiology
Distemper Virus, Canine - genetics
Dogs
Foxes
Genomics
Genotypes
Health risks
Hemagglutinins
Humanities and Social Sciences
multidisciplinary
Nanopores
Phylogeny
Polymerase chain reaction
Science
Science (multidisciplinary)
Strains (organisms)
Technology
Viruses
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Summary:Canine distemper virus (CDV) endangers a wide range of wild animal populations, can cross species barriers and therefore representing a significant conservational and animal health risk around the globe. During spring to autumn 2021, according to our current estimates a minimum of 50 red foxes ( Vulpes vulpes ) died of CDV in Hungary, with CDV lesions. Oral, nasal and rectal swab samples were RT-PCR screened for Canine Distemper Virus from red fox carcasses. To investigate in more detail the origins of these CDV strains, 19 complete genomes were sequenced with a pan-genotype CDV-specific amplicon-based sequencing method developed by our laboratory and optimized for the Oxford Nanopore Technologies platform. Phylogenetic analysis of the complete genomic sequences and separately the hemagglutinin gene sequences revealed the role of the Europe lineage of CDV as a causative agent for the current epizootic. Here we highlight the growing importance of fast developing rapid sequencing technologies to aid rapid response activities during epidemics or epizootic events. We also emphasize the urgent need for improved surveillance of CDV, considering the epizootic capability of enzootic strains as reported in the current study. For such future efforts, we provide a novel NGS protocol to facilitate future genomic surveillance studies.
ISSN:2045-2322
2045-2322
DOI:10.1038/s41598-022-08183-3