Membrane water for probing neuronal membrane potentials and ionic fluxes at the single cell level

Neurons communicate through electrochemical signaling within a complex network. These signals are composed of changes in membrane potentials and are traditionally measured with the aid of (toxic) fluorescent labels or invasive electrical probes. Here, we demonstrate an improvement in label-free seco...

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Bibliographic Details
Published in:Nature communications 2018-12, Vol.9 (1), p.5287-7, Article 5287
Main Authors: Didier, M. E. P., Tarun, O. B., Jourdain, P., Magistretti, P., Roke, S.
Format: Article
Language:English
Subjects:
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Brain
Electric properties
Electrochemistry
Fluorescence
Fluxes
Humanities and Social Sciences
Ion channels
Ion flux
Laboratories
Medical imaging
Membrane potential
multidisciplinary
Neuroimaging
Neurology
Neurons
Physiology
Science
Science (multidisciplinary)
Spatial distribution
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Summary:Neurons communicate through electrochemical signaling within a complex network. These signals are composed of changes in membrane potentials and are traditionally measured with the aid of (toxic) fluorescent labels or invasive electrical probes. Here, we demonstrate an improvement in label-free second harmonic neuroimaging sensitivity by ~3 orders of magnitude using a wide-field medium repetition rate illumination. We perform a side-by-side patch-clamp and second harmonic imaging comparison to demonstrate the theoretically predicted linear correlation between whole neuron membrane potential changes and the square root of the second harmonic intensity. We assign the ion induced changes to the second harmonic intensity to changes in the orientation of membrane interfacial water, which is used to image spatiotemporal changes in the membrane potential and K + ion flux. We observe a non-uniform spatial distribution and temporal activity of ion channels in mouse brain neurons. Non-invasive spatiotemporal probing of electric potentials in living neurons without chemical or genetic modification provides a major advancement to neuroscience. Here, the authors demonstrate the use of membrane water as a probe for neuronal membrane potentials and ionic flux.
ISSN:2041-1723
2041-1723
DOI:10.1038/s41467-018-07713-w