Host Prdx6 contributing to the intracellular survival of Brucella suis S2 strain

• Published in: BMC veterinary research 2019-08, Vol.15 (1), p.304-304, Article 304
• Main Authors: Wang, Lu-Lu, Chen, Xiao-Feng, Hu, Pan, Lu, Shi-Ying, Fu, Bao-Quan, Li, Yan-Song, Zhai, Fei-Fei, Ju, Dan-Di, Zhang, Shi-Jun, Shui, Yi-Ming, Chang, Jiang, Ma, Xiao-Long, Su, Bing, Zhou, Yu, Liu, Zeng-Shan, Ren, Hong-Lin
• Format: Article
• Language: English
• Subjects: Animals; Antibodies; Antioxidants; Apoptosis; Bacterial infections; Brucella; Brucella suis; Brucella suis - physiology; Brucellosis; Brucellosis - microbiology; Communicable diseases; Deoxyribonucleic acid; Disease transmission; DNA; E coli; Experiments; Female; Gene expression; Gene Expression Regulation - physiology; Gene Knockdown Techniques; Genetic aspects; Health aspects; Host; House mouse; Identification and classification; Infections; Infectious diseases; Intracellular; Intracellular survival; Laboratories; Macrophage; Macrophages; Macrophages - metabolism; Mammals; Mice; Mice, Inbred BALB C; Molecular weight; Monoclonal antibodies; Monocytes; Peroxidase; Peroxiredoxin; Peroxiredoxin VI - metabolism; Pets; Phospholipase; Phospholipase A2; Phospholipases; Plasmids; Prdx6; Proteins; RAW 264.7 Cells; Survival; Veterinary medicine; Zoonoses
• ISSN: 1746-6148; 1746-6148
• DOI: 10.1186/s12917-019-2049-8

Brucellosis is a worldwide zoonotic infectious disease that is transmitted in various ways and causes great harm to humans and animals. The brucellosis pathogen is Brucella, which mainly resides in macrophage cells and survives and replicates in host cells. However, the mechanisms underlying Brucella survival in macrophage cells have not been thoroughly elucidated to date. Peroxiredoxin 6 (Prdx6) is a bifunctional protein that shows not only GSH peroxidase activity but also phospholipase A2 activity and plays important roles in combating oxidative damage and regulating apoptosis. Recombinant mouse (Mus musculus) Prdx6 (MmPrdx6) was expressed and purified, and monoclonal antibodies against MmPrdx6 were prepared. Using the Brucella suis S2 strain to infect RAW264.7 murine macrophages, the level of intracellular Prdx6 expression first decreased and later increased following infection. Overexpressing Prdx6 in macrophages resulted in an increase in B. suis S2 strain levels in RAW264.7 cells, while knocking down Prdx6 reduced the S2 levels in cells. Host Prdx6 can increase the intracellular survival of B. suis S2 strain and plays a role in Brucella infection.