The Cap Snatching of Segmented Negative Sense RNA Viruses as a Tool to Map the Transcription Start Sites of Heterologous Co-infecting Viruses

Identification of the transcription start sites (TSSs) of a virus is of great importance to understand and dissect the mechanism of viral genome transcription but this often requires costly and laborious experiments. Many segmented negative-sense RNA viruses (sNSVs) cleave capped leader sequences fr...

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Bibliographic Details
Published in:Frontiers in microbiology 2017-12, Vol.8, p.2519-2519
Main Authors: Lin, Wenzhong, Qiu, Ping, Jin, Jing, Liu, Shunmin, Ul Islam, Saif, Yang, Jinguang, Zhang, Jie, Kormelink, Richard, Du, Zhenguo, Wu, Zujian
Format: Article
Language:English
Subjects:
Cap-snatching
Co-infection
Geminivirus
Microbiology
Rice stripe virus
SNSV
Tenuivirus
TSS
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Summary:Identification of the transcription start sites (TSSs) of a virus is of great importance to understand and dissect the mechanism of viral genome transcription but this often requires costly and laborious experiments. Many segmented negative-sense RNA viruses (sNSVs) cleave capped leader sequences from a large variety of mRNAs and use these cleaved leaders as primers for transcription in a conserved process called cap snatching. The recent developments in high-throughput sequencing have made it possible to determine most, if not all, of the capped RNAs snatched by a sNSV. Here, we show that rice stripe tenuivirus (RSV), a plant-infecting sNSV, co-infects with two different begomoviruses and snatches capped leader sequences from their mRNAs. By determining the 5' termini of a single RSV mRNA with high-throughput sequencing, the 5' ends of almost all the mRNAs of the co-infecting begomoviruses could be identified and mapped on their genomes. The findings in this study provide support for the using of the cap snatching of sNSVs as a tool to map viral TSSs.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2017.02519