Variation and functional profile of gut bacteria in the scarab beetle, Anomala dimidiata, under a cellulose-enriched microenvironment

• Published in: Scientific reports 2024-09, Vol.14 (1), p.22400-15, Article 22400
• Main Authors: Msango, Kondwani, Gouda, M. N. Rudra, Ramakrishnan, B., Kumar, Aundy, Subramanian, Sabtharishi
• Format: Article
• Language: English
• Subjects: 631/326/41/2142; 631/601/1466; Animals; Anomala dimidiata; Bacteria; Bacteria - classification; Bacteria - genetics; Bacteria - isolation & purification; Bacteria - metabolism; Bioprospecting; Biotechnology; Carbohydrate metabolism; Cellulolytic activity; Cellulose; Cellulose - metabolism; Coleoptera - microbiology; Digestive system; Fermentation; Functional profiling; Gastrointestinal Microbiome; Gastrointestinal tract; Gut bacteria; Hindgut; Humanities and Social Sciences; Larva - microbiology; Larvae; Metabolism; Metagenomic analysis; Microenvironments; Midgut; multidisciplinary; Phylogeny; RNA, Ribosomal, 16S - genetics; Science; Science (multidisciplinary); Symbionts; Symbiosis; Taxonomy
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-73417-5

This study utilized cultivable methods and 16 S amplicon sequencing to compare taxonomic profiles and functional potential of gut bacteria in the scarab beetle, Anomola dimidiata , under cellulose-enriched conditions. Eight culturable cellulolytic gut bacteria were isolated from the midgut and hindgut of the scarab larvae, respectively. 16 S amplicon sequencing evinced that the most represented taxonomic profiles at phylum level in the fermentation chamber and midgut were Bacillota (71.62 and 56.76%), Pseudomonadota (22.66 and 36.89%) and Bacteroidota (2.7 and 2.81%). Bacillota (56.74 and 91.39%) were significantly enriched in the midgut with the addition of cellulose. In contrast, Bacillota and Psedomonadota were significantly enriched in the fermentation chamber. Carbohydrate metabolism was up-regulated in the midgut, while nitrogen and phosphorus metabolism were up-regulated in the fermentation chamber, suggesting these symbionts’ possible metabolic roles to the host. An analysis of total cellulases as well as amplicon sequence variants indicated that the gut bacteria belonging to Acinetobacter , Bacillus , Brucella , Brevibacillus , Enterobacter , Lysinibacillus and Paenibacillus are involved in nutrition provisioning. These results have provided additional insights into the gut bacteria associated with cellulose digestion in A. dimidiata and created a platform for bioprospecting novel isolates to produce biomolecules for biotechnological use, besides identifying eco-friendly targets for its management.