RNA Sequencing Analysis Reveals Divergent Adaptive Response to Hypo- and Hyper-Salinity in Greater Amberjack ( Seriola dumerili ) Juveniles

Salinity significantly affects physiological and metabolic activities, breeding, development, survival, and growth of marine fish. The greater amberjack ( ) is a fast-growing species that has immensely contributed to global aquaculture diversification. However, the tolerance, adaptation, and molecul...

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Bibliographic Details
Published in:Animals (Basel) 2022-01, Vol.12 (3), p.327
Main Authors: Peng, Yuhao, Shi, Hongjuan, Liu, Yuqi, Huang, Yang, Zheng, Renchi, Jiang, Dongneng, Jiang, Mouyan, Zhu, Chunhua, Li, Guangli
Format: Article
Language:English
Subjects:
7-Dehydrocholesterol reductase
Adaptation
Aquaculture
Bioinformatics
Biological activity
Biosynthesis
carangidae
Cytokines
differential gene expression
Divergence
Encyclopedias
Enrichment
Fish
Gene expression
Genes
Genomes
Immune response
Immunological tolerance
Insulin
Juveniles
Lipid metabolism
Lipids
Marine fish
Metabolism
Molecular modelling
Physiology
Salinity
Salinity effects
Seawater
Sequence analysis
Seriola dumerili
Signal transduction
Steroids
Stress
Tilapia
Tissue analysis
TLR5 protein
Toll-like receptors
transcriptome
Transcriptomics
Tryptophan
Wnt protein
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Summary:Salinity significantly affects physiological and metabolic activities, breeding, development, survival, and growth of marine fish. The greater amberjack ( ) is a fast-growing species that has immensely contributed to global aquaculture diversification. However, the tolerance, adaptation, and molecular responses of greater amberjack to salinity are unclear. This study reared greater amberjack juveniles under different salinity stresses (40, 30, 20, and 10 ppt) for 30 days to assess their tolerance, adaptation, and molecular responses to salinity. RNA sequencing analysis of gill tissue was used to identify genes and biological processes involved in greater amberjack response to salinity stress at 40, 30, and 20 ppt. Eighteen differentially expressed genes (DEGs) (nine upregulated and nine downregulated) were identified in the 40 vs. 30 ppt group. Moreover, 417 DEGs (205 up-regulated and 212 down-regulated) were identified in the 20 vs. 30 ppt group. qPCR and transcriptomic analysis indicated that salinity stress affected the expression of genes involved in steroid biosynthesis ( , , , , , and ), lipid metabolism ( , , , and ), ion transporters ( , , , and ), and immune response ( and ). Furthermore, KEGG pathway enrichment analysis showed that the DEGs were enriched in steroid biosynthesis, lipids metabolism, cytokine-cytokine receptor interaction, tryptophan metabolism, and insulin signaling pathway. Therefore, this study provides insights into the molecular mechanisms of marine fish adaptation to salinity.
ISSN:2076-2615
2076-2615
DOI:10.3390/ani12030327