Biofilm formation of Staphylococcus aureus on various implants used for surgical treatment of destructive spondylodiscitis

• Published in: Scientific reports 2024-08, Vol.14 (1), p.19364-10, Article 19364
• Main Authors: Zacher, Amrei T., Mirza, Kamran, Thieme, Lara, Nietzsche, Sandor, Senft, Christian, Schwarz, Falko
• Format: Article
• Language: English
• Subjects: 631/326/22; 631/326/2565; 631/326/41; 631/326/46; 631/378/1689; 639/301/54; 692/308; 692/420; 692/617; 692/699; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Antibiotics; Bacteria; Bacterial Adhesion; Benzophenones; Biofilms; Biofilms - growth & development; Bone Cements; Bone growth; Bone implants; Cement; Discitis - microbiology; Discitis - surgery; Extracellular polymers; Humanities and Social Sciences; Humans; Ketones; Microscopic analysis; multidisciplinary; Polyethylene Glycols - chemistry; Polymers - chemistry; Polymethyl methacrylate; Prostheses and Implants - microbiology; Scanning electron microscopy; Science; Science (multidisciplinary); Sonication; Spondylodiscitis; Staphylococcal Infections - microbiology; Staphylococcus aureus - drug effects; Staphylococcus aureus - physiology; Surface area; Tantalum; Titanium; Transplants & implants
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-70244-6

The incidence of spondylodiscitis has witnessed a significant increase in recent decades. Surgical intervention becomes necessary in case of bone destruction to remove infected tissue and restore spinal stability, often involving the implantation of a cage. Despite appropriate treatment, relapses occur in up to 20 percent of cases, resulting in substantial economic and social burdens. The formation of biofilm has been identified as a major contributor to relapse development. Currently, there is no consensus among German-speaking spinal surgeons or in the existing literature regarding the preferred choice of material to minimize relapse rates. Thus, the objective of this study is to investigate whether certain materials used in spinal implants exhibit varying degrees of susceptibility to bacterial attachment, thereby providing valuable insights for improving treatment outcomes.Eight cages of each PEEK, titanium-coated PEEK (Ti-PEEK), titanium (Ti), polyetherketoneketone (PEKK), tantalum (Ta) and antibiotic-loaded bone cement were incubated with 20% human plasma for 24 h. Subsequently, four implants were incubated with S. aureus for 24 h or 48 h each. The biofilm was then removed by sonication and the attained solution plated for Colony Forming Units (CFU) counting. Scanning electron microscopy was used to confirm bacterial attachment. The CFUs have been compared directly and in relation to the cages surface area. The surface area of the implants was PEEK 557 mm 2 , Ti-PEEK 472 mm 2 , Ti 985 mm 2 , PEKK 594 mm 2 , Ta 706 mm 2 , bone cement 123 mm 2 . The mean CFU count per implant and per mm 2 surface area after 24 h and after 48 h was calculated. Bone cement was found to have significantly more CFUs per mm 2  surface area than the other materials tested. When comparing the CFU count per implant, bone cement was statistically significantly more prone to biofilm formation than PEEK after 48 h. There was no statistical significance between the other materials when comparing both CFU count per mm 2 surface area and CFU count per implant. The electron microscopic analysis showed the attachment of the bacteria, as well as production of extracellular polymeric substances (EPS) as a sign for beginning biofilm formation. Antibiotic-loaded bone cement has shown statistically significantly more bacterial attachment than the other examined materials. No difference was found between the other materials regarding bacterial attachment after 24 h and 48 h. Proposed hypothe